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Alkaline Phosphatase–Triggered Immunoassay Based on Fluorogenic Reaction for Sensitive Detection of Acetochlor, Metolachlor, and Propisochlor
Enzyme-linked immunosorbent assay (ELISA), as an immunoassay platform, usually suffers from poor sensitivity due to the limited signal intensity. To overcome this obstacle, an alkaline phosphatase–triggered immunoassay based on fluorogenic reaction of o -phenylenediamine and ascorbic acid was develo...
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| Published in: | Food analytical methods 2020-04, Vol.13 (4), p.1008-1016 |
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| Main Authors: | , , , , , , , , , |
| Format: | Article |
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| cites | cdi_FETCH-LOGICAL-c319t-919a692bd6d408f899d44d3b574965b6fcb7f7a7fb22fcce7b6f0fa6932bbe343 |
| container_end_page | 1016 |
| container_issue | 4 |
| container_start_page | 1008 |
| container_title | Food analytical methods |
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| creator | Zha, Yonghong Liu, Xiaolei Hu, Pan Lu, Shiying Ren, Honglin Liu, Zengshan Liang, Xiongyan Yang, Yuying Li, Yansong Zhou, Yu |
| description | Enzyme-linked immunosorbent assay (ELISA), as an immunoassay platform, usually suffers from poor sensitivity due to the limited signal intensity. To overcome this obstacle, an alkaline phosphatase–triggered immunoassay based on fluorogenic reaction of
o
-phenylenediamine and ascorbic acid was developed for sensitive detection of chloroacetamide herbicides in corn. The new immunoassay revealed limits of detection (LODs) for acetochlor (ATC), metolachlor, and propisochlor of 1.19 ng/mL, 2.34 ng/mL, and 3.40 ng/mL, respectively. The average recoveries of the three chloroacetamide herbicides in corn sample were 88.4%, 91.8%, and 87.6%, respectively. Such fluorescent immunoassay via in situ formation of fluorescent signal from scratch provides a new strategy with excellent sensitivity in point-of-care test of chloroacetamide herbicides. |
| doi_str_mv | 10.1007/s12161-020-01706-6 |
| format | article |
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o
-phenylenediamine and ascorbic acid was developed for sensitive detection of chloroacetamide herbicides in corn. The new immunoassay revealed limits of detection (LODs) for acetochlor (ATC), metolachlor, and propisochlor of 1.19 ng/mL, 2.34 ng/mL, and 3.40 ng/mL, respectively. The average recoveries of the three chloroacetamide herbicides in corn sample were 88.4%, 91.8%, and 87.6%, respectively. Such fluorescent immunoassay via in situ formation of fluorescent signal from scratch provides a new strategy with excellent sensitivity in point-of-care test of chloroacetamide herbicides.</description><identifier>ISSN: 1936-9751</identifier><identifier>EISSN: 1936-976X</identifier><identifier>DOI: 10.1007/s12161-020-01706-6</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Acetochlor ; Alkaline phosphatase ; Analytical Chemistry ; Ascorbic acid ; Chemistry ; Chemistry and Materials Science ; Chemistry/Food Science ; Corn ; Enzyme-linked immunosorbent assay ; Fluorescence ; Food Science ; Herbicides ; Immunoassay ; Metolachlor ; Microbiology ; o-Phenylenediamine ; Pesticides ; Phenylenediamine ; Phosphatase ; Sensitivity ; Sensitivity analysis</subject><ispartof>Food analytical methods, 2020-04, Vol.13 (4), p.1008-1016</ispartof><rights>Springer Science+Business Media, LLC, part of Springer Nature 2020</rights><rights>Springer Science+Business Media, LLC, part of Springer Nature 2020.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c319t-919a692bd6d408f899d44d3b574965b6fcb7f7a7fb22fcce7b6f0fa6932bbe343</citedby><cites>FETCH-LOGICAL-c319t-919a692bd6d408f899d44d3b574965b6fcb7f7a7fb22fcce7b6f0fa6932bbe343</cites><orcidid>0000-0002-5462-1174</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Zha, Yonghong</creatorcontrib><creatorcontrib>Liu, Xiaolei</creatorcontrib><creatorcontrib>Hu, Pan</creatorcontrib><creatorcontrib>Lu, Shiying</creatorcontrib><creatorcontrib>Ren, Honglin</creatorcontrib><creatorcontrib>Liu, Zengshan</creatorcontrib><creatorcontrib>Liang, Xiongyan</creatorcontrib><creatorcontrib>Yang, Yuying</creatorcontrib><creatorcontrib>Li, Yansong</creatorcontrib><creatorcontrib>Zhou, Yu</creatorcontrib><title>Alkaline Phosphatase–Triggered Immunoassay Based on Fluorogenic Reaction for Sensitive Detection of Acetochlor, Metolachlor, and Propisochlor</title><title>Food analytical methods</title><addtitle>Food Anal. Methods</addtitle><description>Enzyme-linked immunosorbent assay (ELISA), as an immunoassay platform, usually suffers from poor sensitivity due to the limited signal intensity. To overcome this obstacle, an alkaline phosphatase–triggered immunoassay based on fluorogenic reaction of
o
-phenylenediamine and ascorbic acid was developed for sensitive detection of chloroacetamide herbicides in corn. The new immunoassay revealed limits of detection (LODs) for acetochlor (ATC), metolachlor, and propisochlor of 1.19 ng/mL, 2.34 ng/mL, and 3.40 ng/mL, respectively. The average recoveries of the three chloroacetamide herbicides in corn sample were 88.4%, 91.8%, and 87.6%, respectively. Such fluorescent immunoassay via in situ formation of fluorescent signal from scratch provides a new strategy with excellent sensitivity in point-of-care test of chloroacetamide herbicides.</description><subject>Acetochlor</subject><subject>Alkaline phosphatase</subject><subject>Analytical Chemistry</subject><subject>Ascorbic acid</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Chemistry/Food Science</subject><subject>Corn</subject><subject>Enzyme-linked immunosorbent assay</subject><subject>Fluorescence</subject><subject>Food Science</subject><subject>Herbicides</subject><subject>Immunoassay</subject><subject>Metolachlor</subject><subject>Microbiology</subject><subject>o-Phenylenediamine</subject><subject>Pesticides</subject><subject>Phenylenediamine</subject><subject>Phosphatase</subject><subject>Sensitivity</subject><subject>Sensitivity analysis</subject><issn>1936-9751</issn><issn>1936-976X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNp9kEFOwzAQRS0EEqVwAVaW2BKwndSOl6VQqFREBUViZzmO3aakcbATpO64AQtuyEkwpIIdq5n58_-M9AA4xugMI8TOPSaY4ggRFCHMEI3oDuhhHtOIM_q0-9sP8D448H6FEEUJJj3wPiyfZVlUGs6W1tdL2UivP98-5q5YLLTTOZys121lpfdyAy_CMoe2guOytc4udFUoeK-laoogGuvgg6580RSvGl7qRne6NXCodGPVsrTuFN6GtpTbQVY5nDlbF75bH4I9I0uvj7a1Dx7HV_PRTTS9u56MhtNIxZg3EcdcUk6ynOYJSk3KeZ4keZwNWMLpIKNGZcwwyUxGiFFKsyAhEyIxyTIdJ3EfnHR3a2dfWu0bsbKtq8JLQeKU8oSylAQX6VzKWe-dNqJ2xVq6jcBIfIMXHXgRwIsf8IKGUNyFfDBXAeLf6X9SX8H9inY</recordid><startdate>20200401</startdate><enddate>20200401</enddate><creator>Zha, Yonghong</creator><creator>Liu, Xiaolei</creator><creator>Hu, Pan</creator><creator>Lu, Shiying</creator><creator>Ren, Honglin</creator><creator>Liu, Zengshan</creator><creator>Liang, Xiongyan</creator><creator>Yang, Yuying</creator><creator>Li, Yansong</creator><creator>Zhou, Yu</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><orcidid>https://orcid.org/0000-0002-5462-1174</orcidid></search><sort><creationdate>20200401</creationdate><title>Alkaline Phosphatase–Triggered Immunoassay Based on Fluorogenic Reaction for Sensitive Detection of Acetochlor, Metolachlor, and Propisochlor</title><author>Zha, Yonghong ; Liu, Xiaolei ; Hu, Pan ; Lu, Shiying ; Ren, Honglin ; Liu, Zengshan ; Liang, Xiongyan ; Yang, Yuying ; Li, Yansong ; Zhou, Yu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c319t-919a692bd6d408f899d44d3b574965b6fcb7f7a7fb22fcce7b6f0fa6932bbe343</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Acetochlor</topic><topic>Alkaline phosphatase</topic><topic>Analytical Chemistry</topic><topic>Ascorbic acid</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Chemistry/Food Science</topic><topic>Corn</topic><topic>Enzyme-linked immunosorbent assay</topic><topic>Fluorescence</topic><topic>Food Science</topic><topic>Herbicides</topic><topic>Immunoassay</topic><topic>Metolachlor</topic><topic>Microbiology</topic><topic>o-Phenylenediamine</topic><topic>Pesticides</topic><topic>Phenylenediamine</topic><topic>Phosphatase</topic><topic>Sensitivity</topic><topic>Sensitivity analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zha, Yonghong</creatorcontrib><creatorcontrib>Liu, Xiaolei</creatorcontrib><creatorcontrib>Hu, Pan</creatorcontrib><creatorcontrib>Lu, Shiying</creatorcontrib><creatorcontrib>Ren, Honglin</creatorcontrib><creatorcontrib>Liu, Zengshan</creatorcontrib><creatorcontrib>Liang, Xiongyan</creatorcontrib><creatorcontrib>Yang, Yuying</creatorcontrib><creatorcontrib>Li, Yansong</creatorcontrib><creatorcontrib>Zhou, Yu</creatorcontrib><collection>CrossRef</collection><jtitle>Food analytical methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zha, Yonghong</au><au>Liu, Xiaolei</au><au>Hu, Pan</au><au>Lu, Shiying</au><au>Ren, Honglin</au><au>Liu, Zengshan</au><au>Liang, Xiongyan</au><au>Yang, Yuying</au><au>Li, Yansong</au><au>Zhou, Yu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Alkaline Phosphatase–Triggered Immunoassay Based on Fluorogenic Reaction for Sensitive Detection of Acetochlor, Metolachlor, and Propisochlor</atitle><jtitle>Food analytical methods</jtitle><stitle>Food Anal. 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o
-phenylenediamine and ascorbic acid was developed for sensitive detection of chloroacetamide herbicides in corn. The new immunoassay revealed limits of detection (LODs) for acetochlor (ATC), metolachlor, and propisochlor of 1.19 ng/mL, 2.34 ng/mL, and 3.40 ng/mL, respectively. The average recoveries of the three chloroacetamide herbicides in corn sample were 88.4%, 91.8%, and 87.6%, respectively. Such fluorescent immunoassay via in situ formation of fluorescent signal from scratch provides a new strategy with excellent sensitivity in point-of-care test of chloroacetamide herbicides.</abstract><cop>New York</cop><pub>Springer US</pub><doi>10.1007/s12161-020-01706-6</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0002-5462-1174</orcidid></addata></record> |
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| ispartof | Food analytical methods, 2020-04, Vol.13 (4), p.1008-1016 |
| issn | 1936-9751 1936-976X |
| language | eng |
| recordid | cdi_proquest_journals_2386946782 |
| source | Springer Nature |
| subjects | Acetochlor Alkaline phosphatase Analytical Chemistry Ascorbic acid Chemistry Chemistry and Materials Science Chemistry/Food Science Corn Enzyme-linked immunosorbent assay Fluorescence Food Science Herbicides Immunoassay Metolachlor Microbiology o-Phenylenediamine Pesticides Phenylenediamine Phosphatase Sensitivity Sensitivity analysis |
| title | Alkaline Phosphatase–Triggered Immunoassay Based on Fluorogenic Reaction for Sensitive Detection of Acetochlor, Metolachlor, and Propisochlor |
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