Differential Diagnostics of Bacteria Using the Surface-Enhanced Raman Spectra

— Surface-enhanced Raman scattering of bioorganic compounds close to silver nanoparticles may be used for species identification of microbial colonies. The preparations of silver nanoparticles sol with a single microbial colony (strains Escherichia coli 376/2, Staphylococcus aureus 25923 , Pseudomon...

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Bibliographic Details
Published in:Microbiology (New York) 2020-03, Vol.89 (2), p.192-196
Main Authors: Borisova, O. V., Galstyan, A. G., Olenin, A. Yu, Lisichkin, G. V., Zverev, V. V.
Format: Article
Language:English
Subjects:
Biomedical and Life Sciences
Colonies
Experimental Articles
Life Sciences
Medical Microbiology
Microbiology
Nanoparticles
Raman spectroscopy
Silver
Species composition
Strains (organisms)
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Summary:— Surface-enhanced Raman scattering of bioorganic compounds close to silver nanoparticles may be used for species identification of microbial colonies. The preparations of silver nanoparticles sol with a single microbial colony (strains Escherichia coli 376/2, Staphylococcus aureus 25923 , Pseudomonas aeruginosa ATCC 27583, and Bacillus subtilis 534) exhibited the individual Raman spectra, in which the band occurrence and intensity correlated with the species composition. The most important differences in the spectra of these four strains were associated with the bands at 649‒652, 948‒950, 1326‒1340, and 1468 cm −1 . Application of the principal component analysis within the range from 600 to 1600 cm −1 resulted in the sum of the factor load of two major components equal to 83.53% of the total differences between the spectra and demonstrated the possibility of differential diagnostics of four microbial species.
ISSN:0026-2617
1608-3237
DOI:10.1134/S002626172001004X