A kinetic approach to assess oxidative metabolism related features in the bivalve Mya arenaria

Electron paramagnetic resonance uses the resonant microwave radiation absorption of paramagnetic substances to detect highly reactive and, therefore, short-lived oxygen and nitrogen centered radicals. Previously, steady state concentrations of nitric oxide, ascorbyl radical (A·) and the labile iron...

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Bibliographic Details
Published in:Theory in biosciences = Theorie in den Biowissenschaften 2012-12, Vol.131 (4), p.253-264
Main Authors: González, Paula Mariela, Abele, Doris, Puntarulo, Susana
Format: Article
Language:English
Subjects:
Animals
Bioinformatics
Biology
Biomedical and Life Sciences
Bivalvia
Complex Systems
Dehydroascorbic Acid - analogs & derivatives
Dehydroascorbic Acid - metabolism
Electron spin resonance
Electron Spin Resonance Spectroscopy
Evolutionary Biology
Free radicals
Invertebrates
Iron - metabolism
Kinetics
Life Sciences
Lipids
Mathematical and Computational Biology
Microwave radiation
Mya - metabolism
Mya arenaria
Nitric oxide
Nitric Oxide - metabolism
North Sea
Original Paper
Oxidative metabolism
Oxidative Stress - physiology
Philosophy of Biology
Superoxide
Superoxides - metabolism
Theoretical Ecology/Statistics
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Summary:Electron paramagnetic resonance uses the resonant microwave radiation absorption of paramagnetic substances to detect highly reactive and, therefore, short-lived oxygen and nitrogen centered radicals. Previously, steady state concentrations of nitric oxide, ascorbyl radical (A·) and the labile iron pool (LIP) were determined in digestive gland of freshly collected animals from the North Sea bivalve Mya arenaria . The application of a simple kinetic analysis of these data based on elemental reactions allowed us to estimate the steady state concentrations of superoxide anion, the rate of A· disappearance and the content of unsaturated lipids. This analysis applied to a marine invertebrate opens the possibility of a mechanistic understanding of the complexity of free radical and LIP interactions in a metabolically slow, cold water organism under unstressed conditions. This data can be further used as a basis to assess the cellular response to stress in a simple system as the bivalve M. arenaria that can then be compared to cells of higher organisms.
ISSN:1431-7613
1611-7530
DOI:10.1007/s12064-012-0159-y