First molecular detection of Lecanosticta acicola from Poland on Pinus mugo

The presence of quarantine pathogen Lecanosticta acicola, the causal agent of brown spot needle blight, was confirmed using molecular methods for the first time in northern part of Poland on the coastal area adjacent to the Baltic Sea. This area includes sandy beaches, where Pinus mugo and P. sylves...

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Bibliographic Details
Published in:Forest pathology = Journal de pathologie forestière = Zeitschrift für Forstpathologie 2020-04, Vol.50 (2), p.n/a
Main Authors: Raitelaitytė, Kristina, Markovskaja, Svetlana, Paulauskas, Algimantas, Hsiang, Tom, Oszako, Tomasz, Andrea, Vannini
Format: Article
Language:English
Subjects:
Blight
Brown spot
Coastal zone
detection
Lecanosticta acicola
Mating
Mating types
Needle blight
Pathogens
PCR
Pine needles
pine pathogen
Pinus mugo
Priming
RT‐PCR
Sequence analysis
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Summary:The presence of quarantine pathogen Lecanosticta acicola, the causal agent of brown spot needle blight, was confirmed using molecular methods for the first time in northern part of Poland on the coastal area adjacent to the Baltic Sea. This area includes sandy beaches, where Pinus mugo and P. sylvestris were planted. Symptomatic needles were collected in 2017 from 20 P. mugo trees growing in one stand in Ustka. Typical symptoms of brown spot needle blight infection, including dead needle tips and central zones with yellow or reddish brown, circular spots in green tissue, were observed on all samples. Only, the asexual stage of L. acicola was obtained during this work. The pathogen species identity was confirmed using classical morphological methods (microscopic examination of the infected needles), real‐time and species‐specific priming (SSPP) PCR, and ITS sequencing. Analysis of mating‐type (MAT) genes showed the presence of both mating types in northern Poland.
ISSN:1437-4781
1439-0329
DOI:10.1111/efp.12589