PEROXIDASE-LINKED ASSAY FOR DETECTION OF ANTIBODIES AGAINST BOVINE LEUKOSIS VIRUS

A peroxidase linked assay (PLA) was designed to screen bovine sera for the presence of specific antibodies against bovine leukosis virus (BLV). Out of 201 samples of bovine sera analyzed, 52.2% were considered positive by PLA, 26.4% by AGID, and 38.9% by ELISA. Western blotting analyses excluded 27...

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Published in:Journal of Immunoassay and Immunochemistry 2013, Vol.34 (4), p.376
Main Authors: de Castro, Clarissa C, Nunes, Cristina F, Finger, Paula F, Siedler, Bianca S, Dummer, Luana, de Lima, Marcelo, Leite, Fábio P L, Fischer, Geferson, Gilberto D'A. Vargas, de O Hübner, Silvia
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Language:English
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Summary:A peroxidase linked assay (PLA) was designed to screen bovine sera for the presence of specific antibodies against bovine leukosis virus (BLV). Out of 201 samples of bovine sera analyzed, 52.2% were considered positive by PLA, 26.4% by AGID, and 38.9% by ELISA. Western blotting analyses excluded 27 samples found to be positive by PLA. PLA showed 100% of sensitivity when compared with AGID and ELISA. Specificity was 64.8% and 78%, respectively (kappa coefficients were 0.70 and 0.83). These findings indicate that PLA can be used as an alternative method for the diagnosis of BLV infection in cattle.