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Gma-miR1508a confers dwarfing, cold tolerance, and drought sensitivity in soybean
Cold stress is a major abiotic factor that inhibits soybean ( Glycine max (Linn.) Merr.) yield. MicroRNAs (miRNAs) are prevalent endogenous gene regulators in eukaryotes that can cleave target messenger RNAs and play essential roles in soybean cold response. In this study, 105 genes, including 22 pe...
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Published in: | Molecular breeding 2020-04, Vol.40 (4), Article 44 |
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Main Authors: | , , , , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | Cold stress is a major abiotic factor that inhibits soybean (
Glycine max
(Linn.) Merr.) yield. MicroRNAs (miRNAs) are prevalent endogenous gene regulators in eukaryotes that can cleave target messenger RNAs and play essential roles in soybean cold response. In this study, 105 genes, including 22 pentatricopeptide repeat (
PPR
) genes and 4 growth-related genes, were predicted to be the target genes of soybean miRNA
miR1508a
(
gma-miR1508a
). The expression of
gma-miR1508a
was induced by cold treatment but inhibited by drought stress. Compared to the wild type (WT), the overexpression of
gma-miR1508a
in soybean plants resulted in dwarfing and thick cell walls.
Gma-miR1508a
transgenic plants exhibited cold tolerance at the germination and young seedling stages, and soluble sugar content was 58.7% higher than that of WT plants under cold stress. The
gma-miR1508a
transgenic lines showed lower survival rates and greater leaf water loss compared to the WT under drought treatment, which indicated that
gma-miR1508a
reduced drought resistance in soybean. Transcript abundances of 2
PPR
and 4 growth-related predicted target genes decreased in
gma-miR1508a
transgenic plants. In the above six genes, the expression patterns of
Glyma.16G162100
(
PPR
) and
Glyma.17G065400
(xyloglucan endo-trans-glucosylase/hydrolase,
XTH
) showed opposite trends with
gma-miR1508a
under cold conditions. 5′-Rapid amplification of complementary DNA ends (5′-RACE) assays of
Glyma.16G162100
revealed that the cleavage sites of
gma-miR1508a
are located at the 15th and 21st nucleotides of the complementary region. This study demonstrated that
gma-miR1508a
confers dwarfing, cold tolerance, and drought sensitivity in soybean. |
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ISSN: | 1380-3743 1572-9788 |
DOI: | 10.1007/s11032-020-01116-w |