The Role of Autophagy in Interferon/Ribavirin Responders and Non-Responders with Hepatitis C Virus Infection

Background: Hepatitis C virus (HCV) is a significant cause of chronic inflammatory liver diseases. HCV infection is a common disorder worldwide, which has become a significant public health concern. The survival of viruses in host cells is associated with their ability to engage in cellular mechanis...

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Published in:Jundishapur journal of microbiology 2020-03, Vol.13 (3), p.1-5
Main Authors: Abbasi, Fahimeh, Bamdad, Taravat, Ghadimi-Moghadam, Abdolkarim, Chubin, Hamzeh, Jamalidoust, Marzieh
Format: Article
Language:English
Subjects:
Antiviral drugs
Autophagy
Hepatitis
Hepatitis C
Infections
Infectious diseases
Interferon
Phagocytosis
Proteins
Public health
Ribavirin
Software
Viral infections
Viruses
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Summary:Background: Hepatitis C virus (HCV) is a significant cause of chronic inflammatory liver diseases. HCV infection is a common disorder worldwide, which has become a significant public health concern. The survival of viruses in host cells is associated with their ability to engage in cellular mechanisms benefitted from.Objectives: This study aimed at evaluating the expression rate of Beclint, as a critical protein of autophagy, and its correlation with interferon-alpha (IFN-a) expression in both IFN-ribavirin responder and non-responder groups.Methods: In this study, a total of 40 samples of the peripheral blood mononuclear cells (PBMCs) were evaluated. Twenty samples were collected from the patients with chronic HCV as non-responders and 20 samples obtained from the patients considered as responders; all samples were collected in the "pretreatment period". The expression level of IFN-a and Beclin 1 mRNAwas assessed byTaq-man real-time PCR.Results: The mean of HCV load was 4.2 x 106 ± 8.8 x 106 and 1.1 x 107 ± 9.1 x 106 in the responder and non-responder groups, respectively. The expression rate of IFN-a in the responder group was significantly higher, than the non-responders (P < 0.02), whereas the expression rate of the Beclin 1 gene was significantly lower in responders compared with non-responders (P< 0.02).Conclusions: In non-responders, the level of Beclin 1 expression and its correlation with IFN-a expression level, along with other genetic and physiological factors of the host, can be considered as influential factors involved in IFN-aexpression, as an antiviral agent.
ISSN:2008-3645
2008-4161
DOI:10.5812/jjm.92560