Feasibility of exhausted sugar beet pulp as raw material for lactic acid production

• Published in: Journal of the science of food and agriculture 2020-05, Vol.100 (7), p.3036-3045
• Main Authors: Díaz, Ana Belén, González, Claudia, Marzo, Cristina, Caro, Ildefonso, Blandino, Ana
• Format: Article
• Language: English
• Subjects: Acid production; Batch Cell Culture Techniques - methods; Beta vulgaris - chemistry; Beta vulgaris - metabolism; Beta vulgaris - microbiology; Bioreactors - microbiology; Calcium carbonate; Culture Media - analysis; Culture Media - metabolism; fed‐batch SSF; Fermentation; Hydrogen-Ion Concentration; Hydrolysis; Lactic acid; Lactic Acid - metabolism; Lactobacillus casei - growth & development; Lactobacillus casei - metabolism; Nutrients; pH control; pH effects; Plant Tubers - chemistry; Plant Tubers - metabolism; Plant Tubers - microbiology; Productivity; Pulp; Saccharification; simultaneous saccharification and fermentation; sugar beet pulp; Sugar beets; Sugar industry; Sugars - chemistry; Sugars - metabolism; Supplements; Waste Products - analysis
• ISSN: 0022-5142; 1097-0010
• DOI: 10.1002/jsfa.10334

BACKGROUND Exhausted sugar beet pulp pellets (ESBPP), a sugar industry by‐product generated after sugar extraction in the sugar production process, have been used as a raw material for lactic acid (LA) production via hydrolysis and fermentation by Lactobacillus casei. To design a more cost‐effective process, simultaneous saccharification and fermentation (SSF) of ESBPP is proposed in the present study. The effects of pH control, nutrient supplementation and solid addition in fed‐batch SSF on lactic acid production were investigated. RESULTS The highest LA concentration (26.88 g L–1) was reached in fed‐batch SSF at a solid/liquid loading of 0.2 g mL–1, with pH control (by adding 30 g L–1 CaCO3 to the medium) and nutrient supplementation (by adding 20 mL of MRS medium per 100 mL of buffer). Under these conditions, a maximum productivity of 0.63 g L−1 h−1 was achieved, which is 2.7 times higher than that attained in the control experiment (SSF inoculated at time 0 h). However, a slightly lower LA yield was obtained, revealing the need of an increasing dose of enzymes at high solid loading SSF. CONCLUSION An efficient fed‐batch SSF strategy with pH control and MRS supplementation is described in the present study, attaining higher LA productivity compared to separate hydrolysis and fermentation and SSF. © 2020 Society of Chemical Industry