Identification of immunomodulatory peptides from zein hydrolysates

Food protein-derived bioactive peptides have been recognized as a promising candidate for the development of functional foods or nutraceuticals. Peptides exhibiting antioxidant, anti-proliferative and anti-hypertensive activities have been characterized from corn proteins while the knowledge on the...

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Bibliographic Details
Published in:European food research & technology 2020-05, Vol.246 (5), p.931-937
Main Authors: Liu, Ping, Liao, Wang, Qi, Xingpu, Yu, Wenlin, Wu, Jianping
Format: Article
Language:English
Subjects:
Agriculture
Analytical Chemistry
Antihypertensives
Antioxidants
Biotechnology
Chemistry
Chemistry and Materials Science
Corn
Food Science
Forestry
Fractionation
Functional foods & nutraceuticals
High-performance liquid chromatography
Hydrolysates
Immunomodulation
Interleukin 6
Interleukins
Liquid chromatography
Macrophages
Original Paper
Peptides
Proteins
Thermolysin
Zein
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Summary:Food protein-derived bioactive peptides have been recognized as a promising candidate for the development of functional foods or nutraceuticals. Peptides exhibiting antioxidant, anti-proliferative and anti-hypertensive activities have been characterized from corn proteins while the knowledge on the presence of immunomodulatory peptides in corn proteins is limited. The objective of this study was to identify the immunomodulatory peptides from corn proteins using interleukin 6 (IL-6) in human macrophage-like U937 cells as the marker. Results indicated that zein protein hydrolysate digested by thermolysin showed a high yield of peptides with potent activity, which was then used for further fractionation by RP-HPLC. We identified five peptides using LC–MS/MS, among which, peptide FLPFNQL showed the highest activity reducing IL-6 production by 57.7% ± 6.1%.
ISSN:1438-2377
1438-2385
DOI:10.1007/s00217-020-03450-x