Effect of temperature on growth, gene expression, and aflatoxin production by Aspergillus nomius isolated from Brazil nuts

• Published in: Mycotoxin research 2020-05, Vol.36 (2), p.173-180
• Main Authors: Yunes, Nathália B. S., Oliveira, Rodrigo C., Reis, Tatiana A., Baquião, Arianne C., Rocha, Liliana O., Correa, Benedito
• Format: Article
• Language: English
• Subjects: Abiotic factors; Aflatoxins; Aflatoxins - biosynthesis; Aspergillus; Aspergillus - genetics; Aspergillus - growth & development; Aspergillus - metabolism; Bertholletia - microbiology; Biomedical and Life Sciences; Biosynthetic Pathways; Chemistry/Food Science; Colonization; Contamination; Environmental factors; Food Microbiology; Fungal Proteins - genetics; Fungi; Gene Expression; Life Sciences; Medical Microbiology; Medicine/Public Health; Microbiology; Nuts; Original Article; Species; Temperature; Temperature effects
• ISSN: 0178-7888; 1867-1632
• DOI: 10.1007/s12550-019-00380-w

Aspergillus nomius is a potent producer of aflatoxins B and G and is one of the most common species of fungi found in Brazil nuts. Temperature is considered a major abiotic factor that influences fungal colonization and aflatoxin production in nuts during pre- and post-harvest. Therefore, assessment of the response of aflatoxigenic species to different temperatures is important to add information about the understanding of aflatoxin production by Aspergillus nomius and may help in the development of new strategies to prevent aflatoxin contamination. The aim of this study was to evaluate the effect of temperature (25, 30, and 35 °C) on the radial growth, aflatoxin production (B and G), and aflatoxin gene expression of seven A. nomius strains isolated from Brazil nuts. The optimal temperature for growth was 30 °C and was also the best condition for the expression of the aflR , aflD , and aflQ genes. However, maximum production of aflatoxins B and G occurred at 25 °C. Interestingly, high expression of the structural gene aflQ was observed in the maximum aflatoxin production condition (25 °C). The present study demonstrates that temperature may influence aflatoxin production by A. nomius . The combination of molecular and physiological data aids the understanding of the aflatoxigenic species response to different temperatures and can assist in predicting the driving environmental factors that influence aflatoxin contamination of Brazil nuts.