Emulsion PCR Amplification of DNA Libraries with Degenerate Central Regions for Aptamer Selection

For emulsion PCR amplification of oligonucleotide combinatorial libraries the conditions for the stable generation of water-in-oil emulsions with drops of optimal size were found, which included the use of 3% ABIL EM 180 silicone emulsifier in the presence of 0.5% Triton Х100 in mineral oil and cont...

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Bibliographic Details
Published in:Russian journal of bioorganic chemistry 2020-03, Vol.46 (2), p.264-268
Main Authors: Kuznetsova, V. E., Shershov, V. E., Miftahov, R. A., Lapa, S. A., Zasedatelev, A. S., Timofeev, E. N., Chudinov, A. V.
Format: Article
Language:English
Subjects:
Amplification
Biochemistry
Biomedical and Life Sciences
Biomedicine
Bioorganic Chemistry
Combinatorial analysis
Emulsions
Letter to the Editor
Life Sciences
Mineral oils
Organic Chemistry
Silicones
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Summary:For emulsion PCR amplification of oligonucleotide combinatorial libraries the conditions for the stable generation of water-in-oil emulsions with drops of optimal size were found, which included the use of 3% ABIL EM 180 silicone emulsifier in the presence of 0.5% Triton Х100 in mineral oil and controlled mechanical mixing.
ISSN:1068-1620
1608-330X
DOI:10.1134/S1068162020020120