Self‐Assembly of DNA–Peptide Supermolecules: Coiled‐Coil Peptide Structures Templated by d‐DNA and l‐DNA Triplexes Exhibit Chirality‐Independent but Orientation‐Dependent Stabilizing Cooperativity

DNA nanostructures have been designed and used in many different applications. However, the use of nucleic acid scaffolds to promote the self‐assembly of artificial protein mimics is only starting to emerge. Herein five coiled‐coil peptide structures were templated by the hybridization of a d‐DNA tr...

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Bibliographic Details
Published in:Chemistry : a European journal 2020-05, Vol.26 (25), p.5676-5684
Main Authors: Lou, Chenguang, Boesen, Josephine Tuborg, Christensen, Niels Johan, Sørensen, Kasper K., Thulstrup, Peter W., Pedersen, Martin Nors, Giralt, Ernest, Jensen, Knud J., Wengel, Jesper
Format: Article
Language:English
Subjects:
Assembly
Chemistry
Chirality
Coils
Deoxyribonucleic acid
DNA
Electrophoresis
Gel electrophoresis
Hybridization
Nanotechnology
Nanotechnology devices
Nucleic acids
oligonucleotide triplexes
peptide coiled-coils
peptide-oligonucleotide conjugates
Peptides
Polarity
Proteins
Scaffolds
sequence polarity
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Summary:DNA nanostructures have been designed and used in many different applications. However, the use of nucleic acid scaffolds to promote the self‐assembly of artificial protein mimics is only starting to emerge. Herein five coiled‐coil peptide structures were templated by the hybridization of a d‐DNA triplex or its mirror‐image counterpart, an l‐DNA triplex. The self‐assembly of the desired trimeric structures in solution was confirmed by gel electrophoresis and small‐angle X‐ray scattering, and the stabilizing synergy between the two domains was found to be chirality‐independent but orientation‐dependent. This is the first example of using a nucleic acid scaffold of l‐DNA to template the formation of artificial protein mimics. The results may advance the emerging POC‐based nanotechnology field by adding two extra dimensions, that is, chirality and polarity, to provide innovative molecular tools for rational design and bottom‐up construction of artificial protein mimics, programmable materials and responsive nanodevices. Artificial protein mimics: The self‐assembly of five peptide coiled‐coil motifs was templated by the hybridization of a d‐DNA triplex or its mirror‐image counterpart, an l‐DNA triplex. The stabilizing cooperation between the two biomolecular domains was shown to be chirality‐independent, but orientation‐dependent.
ISSN:0947-6539
1521-3765
DOI:10.1002/chem.201905636