Bacterial diversity in the rhizosphere of a transgenic versus a conventional maize (Zea mays)

Genetically modified crops could cause negative effects on bacterial communities. In this study, we compared the bacterial community structure of two maize cultivars to determine whether the transgenic cultivar exerts a negative effect on bacterial communities inhabiting the rhizosphere. Cultivars i...

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Bibliographic Details
Published in:Phyton (Buenos Aires) 2016, Vol.85 (1), p.210-217
Main Authors: L, Vital-L髉ez, Cruz-Hern醤dez, MA, Fern醤dez-D醰ila, S, Mendoza-Herrera, A
Format: Article
Language:English
Subjects:
Amplification
Bacteria
Community structure
Conformation
Corn
Cultivars
Deoxyribonucleic acid
DNA
Gene polymorphism
Genetic modification
Genetically engineered microorganisms
Glufosinate
Herbicide resistance
Herbicides
Metagenomics
Microorganisms
Molecular conformation
Phosphinothricin
Polymerase chain reaction
Polymorphism
Rhizosphere
rRNA
Zea mays
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Summary:Genetically modified crops could cause negative effects on bacterial communities. In this study, we compared the bacterial community structure of two maize cultivars to determine whether the transgenic cultivar exerts a negative effect on bacterial communities inhabiting the rhizosphere. Cultivars included the genetically modified maize (Zea mays), with the pat-gene conferring resistance to the herbicide glufosinate (synonym: L-phosphinothricin), and the hybrid, conventional maize. Metagenomic DNA was extracted from the rhizosphere of plants grown in a greenhouse. Single-strand conformation polymorphism, based on polymerase chain reaction amplifying a partial subunit rRNA gene was used to characterize and generate genetic profiles that corresponded to the bacterial communities of the amplified products from the rhizosphere of the two maize cultivars. Genetic profiles of the rhizospheres consisted of distinguishable profiles, based on the chosen primer pairs. Similarity analyses of patterns found by binary matrix analyses showed no differences in the bacterial communities of the two cultivars. This analysis showed that the microbial population’s structures of the conventional and genetically modified maize were very homogeneous. Genetic modification did not adversely affect the structural bacterial community in the rhizosphere of the transgenic maize cultivar.
ISSN:1851-5657
0031-9457
1851-5657
DOI:10.32604/phyton.2016.85.210