Genetic variability of nanche in Tabasco, Mexico, determined with RAPDs

Nanche (Byrsonima crassifolia (L.) H.B.K.) grows in the southeastern region of the Mexican republic under both wild and semi-cultivated forms. Despite the importance of the fruit of this tree, only one paper at the molecular level has been published in Mexico, and possibly in America, to date. For t...

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Bibliographic Details
Published in:Phyton (Buenos Aires) 2013, Vol.82 (1), p.209-214
Main Authors: ME, Mart韓ez, Lesher G, JM, Casta耋n N, G, de la Cruz L, E, Zapata H, C
Format: Article
Language:English
Subjects:
Amplification
Byrsonima crassifolia
Correlation coefficient
Correlation coefficients
Deoxyribonucleic acid
DNA
Genetic analysis
Genetic diversity
Genetic variability
Germplasm
Oligonucleotides
Polymerase chain reaction
Polymorphism
Random amplified polymorphic DNA
Variance analysis
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Summary:Nanche (Byrsonima crassifolia (L.) H.B.K.) grows in the southeastern region of the Mexican republic under both wild and semi-cultivated forms. Despite the importance of the fruit of this tree, only one paper at the molecular level has been published in Mexico, and possibly in America, to date. For this reason, the purpose of this research was to study the genetic variability of nanche trees that grow under both wild and semi-cultivated conditions in the state of Tabasco, Mexico. Molecular determinations were carried out in the Laboratorio de Genómica of the División Académica de Ciencias Biológicas (DACBiológicas), Universidad Juárez Autónoma de Tabasco (UJAT), using the RAPD (Random Amplified Polymorphic DNA) technique. Of the six evaluated oligonucleotides, only four amplified 10.3 bands each one on average, and of these, three presented polymorphism (2,8 polymorphic bands). Thus, we consider convenient to use other primers or molecular markers in future studies on nanches. Dice´s analysis detected six groups, and the cophenetic correlation coefficient was r=0.82, indicating a good formation of groups of the evaluated material. The average genetic diversity (Hi) presented a moderate value (0.12). According to the analysis of molecular variance (AMOVA), genetic diversity was greater within (85.20%) than among populations (14.80%), and the fixation index (Fst) was 0.148. These results indicate that the RAPD technique was useful for identify the variation of the Byrsonima crassifolia (L.) H.B.K., and this is very valuable for the in situ maintenance of a nanche germplasm bank.
ISSN:1851-5657
0031-9457
1851-5657
DOI:10.32604/phyton.2013.82.209