Estimation of the genetic diversity of Opuntia spp. using molecular markers AFLP

The objective of this study was to apply the AFLP-type molecular marker technique to estimate genetic diversity in cactus pear within the Germplasm Bank of FAUANL. Twelve accessions have been reported as possible duplicates through type RAPD- molecular markers in such Bank. DNA was extracted using t...

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Bibliographic Details
Published in:Phyton (Buenos Aires) 2009-01, Vol.78, p.117
Main Authors: García-Zambrano Ea, Zavala-García, F, Gutiérrez-Diez, A, Ojeda-Zacarías, Mc, Cerda-Hurtado, I
Format: Article
Language:eng ; fre ; ger ; ita ; por ; spa
Subjects:
Amplification
Amplified fragment length polymorphism
Deoxyribonucleic acid
DNA
Genetic distance
Genetic diversity
Germplasm
Markers
Random amplified polymorphic DNA
Reproduction (copying)
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Summary:The objective of this study was to apply the AFLP-type molecular marker technique to estimate genetic diversity in cactus pear within the Germplasm Bank of FAUANL. Twelve accessions have been reported as possible duplicates through type RAPD- molecular markers in such Bank. DNA was extracted using the cellular ruptor technique, and then digested and bound. A preamplification and subsequent selective amplification were conducted. Amplified fragments were then separated and analyzed. It was concluded that none of the accessions was duplicated. This was because 8 groups were formed in the dendogram obtained after applying the UPGMA method. There were 3 accessions in each of two groups. The six remaining groups were formed by one accession each. These results were confirmed when the genetic distance was estimated among the study accessions, since none of them gave the zero value.
ISSN:0031-9457
1851-5657