Inulinase Production by Agro-Industrial Residues: Optimization of Pretreatment of Substrates and Production Medium

• Published in: Food and bioprocess technology 2009-12, Vol.2 (4), p.409-414
• Main Authors: Sguarezi, Carla, Longo, Carine, Ceni, Gustavo, Boni, Gabriela, Silva, Marceli F, Di Luccio, Marco, Mazutti, Marcio A, Maugeri, Francisco, Rodrigues, Maria Isabel, Treichel, Helen
• Format: Article
• Language: English
• Subjects: Acidity; Agricultural industry; Agriculture; Antioxidants; Ascorbic acid; Biotechnology; Chemistry; Chemistry and Materials Science; Chemistry/Food Science; Chitosan; corn steep liquor; culture media; Diffraction patterns; enzyme substrates; Epigallocatechin gallate; Experimental design; Fermentation; Food Science; Fourier transforms; Free radicals; Fruit juices; Grafting; Grapes; Hydrogen bonding; Hydrogen bonds; hydrolysates; Infrared spectroscopy; inulinase; Juices; Kluyveromyces marxianus; Microorganisms; molasses; NMR; Nuclear magnetic resonance; Original Paper; Phenolic compounds; phosphoric acid; Preservation; processing residues; processing waste; response surface methodology; Sensory properties; Sugarcane; Sulfuric acid; Thermal stability; Thermodynamic properties; waste liquors; X-ray diffraction; Yeasts
• ISSN: 1935-5130; 1935-5149
• DOI: 10.1007/s11947-007-0042-x

In this work, production of inulinase was studied. Media formulation was optimized by experimental design and response surface techniques, as well as the pretreatment of the agro-industry residues used in the formulation of fermentation medium. Two agro-industry residues were investigated: sugarcane molasses (SCM) and corn steep liquor (CSL). Pretreatment with sulfuric acid was the most effective for clarification of SCM (pH 5.0, 24 h of resting time and final pH 4.0). Clarification of CSL was accomplished with phosphoric acid (pH 3.0, 24 h of resting time and final pH 5.5). A color reduction of approximately 70% was achieved for both substrates. The highest production of inulinase was obtained in a medium containing 100 g l⁻¹ of pretreated SCM, 100 g l⁻¹ of pretreated CSL and 6 g l⁻¹ of Prodex Lac (yeast hydrolysate), yielding 1,139 U ml⁻¹.