Optimization of physicochemical parameters of tannase post-purification and its versatile bioactivity

The present study investigates the optimization of tannase production from Aspergillus nidulans for various physicochemical parameters and harvests tannase for its chemical characterization. The maximum tannase activity was observed on the third day of incubation at 35°C and the stability was observ...

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Bibliographic Details
Published in:FEMS microbiology letters 2018-06, Vol.365 (12)
Main Authors: Hidayathulla, Syed, Shahat, Abdelaaty A, Alsaid, Mansour S, Al-Mishari, Abdullah A
Format: Article
Language:English
Subjects:
Ammonium
Ammonium sulfate
Antibacterial activity
Antibiotics
Antimicrobial agents
Antioxidants
Benzene
Biofilms
Biological activity
Ceftazidime
Gel electrophoresis
Hydroxyl groups
Microbiology
Microorganisms
Optimization
Parameters
Physicochemical properties
Purification
Ring structures
Scavenging
Sodium lauryl sulfate
Streptomycin
Sulfates
Tannase
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Summary:The present study investigates the optimization of tannase production from Aspergillus nidulans for various physicochemical parameters and harvests tannase for its chemical characterization. The maximum tannase activity was observed on the third day of incubation at 35°C and the stability was observed at pH 5.5-6.0 by holding its 100% activity. The tannase was partially purified from A. nidulans [FT10] by ammonium sulfate precipitation at different concentrations, and it was found that at 80% of ammonium sulfate concentration, the precipitate exhibited the maximum activity for tannase of 96 U/ml. LCMS showed its M/Z value as 162.3 which was reconfirmed by SDS-PAGE. The UV spectrum and FTIR confirmed the presence of two oxy- and three hydroxyl groups in the benzene ring structure. The antibacterial activity of tannase was enhanced with antibiotics such as streptomycin and ceftazidime whereas the biofilm formation was significantly inhibited by the purified tannase. The scavenging activity was greatly increased with purified component and when the concentration of the purified tannase, FT10 was increased. To the best of our knowledge, this is one of the few reports where microbial species was used as the source for producing tannase enzyme and its role in various bioactivities such as antibacterial, anti-biofilm and antioxidant activity was evaluated.
ISSN:1574-6968
0378-1097
1574-6968
DOI:10.1093/femsle/fny051