Duodenogastric Reflux Increases the Penetration of N‐ 3 H‐Methyl‐N‐nitro‐N‐nitrosoguanidine into the Antral Mucosa of Rats: A Possible Role for Mucosal Erosions and Increased Cell Proliferation in Gastric Carcinogenesis

Duodenogastric reflux is a risk factor for gastric carcinogenesis, but the pathogenesis is not fully understood. We studied the risk of N‐methyl‐N‐nitro‐N‐nitrosoguanidine (MNNG)‐induced carcinogenesis in the antrum of rats with duodenogastric reflux. Duodenal fluid was directed into the stomach thr...

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Bibliographic Details
Published in:Cancer science 2002-05, Vol.93 (5)
Main Authors: Øvrebø, Kjell K, Svanes, Knut, Aase, Steinar, Grong, Ketil, Sørbye, Halfdan
Format: Article
Language:English
Subjects:
Autoradiography
Carcinogenesis
Cell growth
Cell proliferation
Gastric cancer
Immunohistochemistry
Mucosa
Risk factors
Stomach
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Summary:Duodenogastric reflux is a risk factor for gastric carcinogenesis, but the pathogenesis is not fully understood. We studied the risk of N‐methyl‐N‐nitro‐N‐nitrosoguanidine (MNNG)‐induced carcinogenesis in the antrum of rats with duodenogastric reflux. Duodenal fluid was directed into the stomach through the pylorus (pyloric reflux group) or through a gastrojejunostomy (jejunal reflux group). After twenty‐four weeks, 5‐bromo‐2‐deoxyuridine (BrdU) was injected intravenously and the stomach was exposed to N‐3H‐methyl‐N‐nitro‐N‐nitrosoguanidine (3H‐MNNG). The antral mucosa was examined with immunohistochemistry and autoradiography for identification of proliferating cells (BrdU labelled) and cells at risk of MNNG‐induced carcinogenesis (3H‐MNNG and BrdU‐labelled cells). Duodenogastric reflux increased the number of double‐labelled cells in the antral mucosa from 4.8±0.6 per mm in the control group to 11.3±1.9 in the jejunal reflux group (P
ISSN:1347-9032
1349-7006