Magnetic Cross-Linked Enzyme Aggregates of Aspergillus oryzae ST11 Lipase Using Polyacrylonitrile Coated Magnetic Nanoparticles for Biodiesel Production

The biodiesel production by enzymatic catalysis is the environmentally friendly production process. In this work, the polyacrylonitrile coated magnetic nanoparticles were prepared and used as a core supporter for producing magnetic cross-linked enzyme aggregates (mCLEAs) from Aspergillus oryzae ST11...

Full description

Saved in:
Bibliographic Details
Published in:Applied biochemistry and biotechnology 2020-04, Vol.190 (4), p.1319-1332
Main Authors: Paitaid, Pattarapon, H-Kittikun, Aran
Format: Article
Language:English
Subjects:
Acrylic Resins - chemistry
Aggregates
Animals
Aspergillus oryzae
Aspergillus oryzae - enzymology
Biocatalysis
Biochemistry
Biodiesel fuels
Biofuels
Biotechnology
Bovine serum albumin
Catalysis
Cattle
Chemistry
Chemistry and Materials Science
Conversion
Crosslinking
Diesel
Enzymes
Enzymes - chemistry
Enzymes, Immobilized - chemistry
Hydrogen-Ion Concentration
Lipase
Lipase - chemistry
Magnetics
Magnetite Nanoparticles - chemistry
Methanol
Moisture content
Nanoparticles
Polyacrylonitrile
Serum albumin
Serum Albumin, Bovine - chemistry
Spectrophotometry, Infrared
Temperature
Water content
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The biodiesel production by enzymatic catalysis is the environmentally friendly production process. In this work, the polyacrylonitrile coated magnetic nanoparticles were prepared and used as a core supporter for producing magnetic cross-linked enzyme aggregates (mCLEAs) from Aspergillus oryzae ST11 lipase with the co-feeding of bovine serum albumin (BSA). The highest immobilized lipase activity was 0.09 U/mg-support or 81.7% of recovered activity. The resulting mCLEAs exhibited the desired characteristics; it had improved the stabilities for pH, temperature, as well as storage comparing to free enzyme. Moreover, when this biocatalyst was used for biodiesel production at the optimal conditions (30% w/w of mCLEAs, 30% w/w of water content and stepwise addition of methanol), the biodiesel conversion was 95% within 24 h of reaction, while one-step addition of methanol produced 81% conversion. The mCLEAs could be reused for 5 cycles and retained the biodiesel conversion higher than 60%.
ISSN:0273-2289
1559-0291
DOI:10.1007/s12010-019-03196-7