Biosynthesis of hydrazine from ammonium and hydroxylamine using an anaerobic ammonium oxidizing bacterium

• Published in: Biotechnology letters 2020-06, Vol.42 (6), p.979-985
• Main Authors: Oshiki, Mamoru, Yamada, Koshiro, Kato, Itsuki, Okoshi, Kento, Imai, Toshiro, Yamaguchi, Takashi, Araki, Nobuo
• Format: Article
• Language: English
• Subjects: Ammonia-oxidizing bacteria; Ammonium; Ammonium Compounds - metabolism; Anaerobiosis - physiology; Applied Microbiology; Bacteria; Bacteria - metabolism; Biochemistry; Biological activity; Biomedical and Life Sciences; Bioreactors - microbiology; Biosynthesis; Biotechnology; Candidatus Kuenenia stuttgartiensis; Cultivation; Feed systems; Hydrazine; Hydrazines; Hydrazines - metabolism; Hydrogen-Ion Concentration; Hydroxylamine; Hydroxylamine - metabolism; Life Sciences; Microbiology; Original Research Paper; Oxidation; Oxidation-Reduction; pH effects; Temperature
• ISSN: 0141-5492; 1573-6776
• DOI: 10.1007/s10529-020-02865-6

Objectives To synthesize hydrazine (N 2 H 4 ) from ammonium and hydroxylamine (NH 2 OH) using an anaerobic ammonium oxidation (anammox) bacterium, Candidatus Kuenenia stuttgartiensis . Results K . stuttgartiensis cells were anoxically cultivated with the addition of ammonium (2 mM) and NH 2 OH (1–100 mM) at pH 6–10.5, and 4–65 °C to examine the favorable cultivation conditions for N 2 H 4 production. The influence of NH 2 OH concentration was more prominent than that of pH and temperature, and NH 2 OH concentration higher than 1 mM deteriorated N 2 H 4 yields significantly. The following conditions were found to be favorable for N 2 H 4 production using K . stuttgartiensis cells: pH 9, 38 °C, and