Biochemical Properties of Carbohydrate-Active Enzymes Synthesized by Penicillium chrysogenum Using Corn Straw as Carbon Source

• Published in: Waste and biomass valorization 2020-06, Vol.11 (6), p.2455-2466
• Main Authors: Silva, Luísa de M. B., Gomes, Tainah C., Ullah, Sadia F., Ticona, Alonso R. P., Hamann, Pedro R. V., Noronha, Eliane F.
• Format: Article
• Language: English
• Subjects: Acids; Carbohydrates; Carbon; Carbon sources; Cell walls; Cellobiase; Corn; Corn straw; Coumaric acid; Engineering; Environment; Environmental Engineering/Biotechnology; Enzymes; Ferulic acid; Fungi; Glucosidase; Hydrolysis; Industrial applications; Industrial Pollution Prevention; Lignin; Lignocellulose; Original Paper; p-Coumaric acid; Pectinase; Penicillium chrysogenum; pH effects; Pretreatment; Renewable and Green Energy; Savannahs; Straw; Syringaldehyde; Thermal stability; Waste Management/Waste Technology; β-Glucosidase
• ISSN: 1877-2641; 1877-265X
• DOI: 10.1007/s12649-019-00589-x

Lignocellulosic material is an alternative, renewable and cheaper source of molecules to be applied in greener industrial processes. Its utilization for this purpose requests steps of pre-treatment and hydrolysis. Filamentous fungi are receiving attention as source of plant cell wall degrading enzymes to apply in lignocellulosic biomass hydrolysis. In the present study, a strain of Penicillium chrysogenum CCDCA10756 isolated from Brazilian Cerrado soil (Savannah like biome) was evaluated as a producer of plant cell wall degrading enzymes aiming industrial application. The fungus cultivated in the presence of corn straw as sole carbon source secreted cellulases (endo-β-1,4-glucanases, cellobiohydrolases, β-glucosidases), endo-β-1,4-xylanases, and pectinases. Endo-β-1,4-xylanases and pectinases presented earlier production reaching maximum values after 3 days of growth in comparison to cellulolytic activities mostly produced after 5 days. Cellobiohydrolases and endo-β-1,4-glucanases present maximal activity in acid pH (3 and 4) and at 50 °C, whereas β-glucosidase presents maximal activity at pH 5.0 and 60 °C. Pectinases showed maximum activity in pH 8 at 50 °C. Furthermore, endo-β-1,4-glucanases and cellobiohydrolases displayed remarkable thermostability at 40 °C. Lignin-derived compounds, trans-ferulic acid, 4-hydroxybenzoic and syringaldehyde inhibited cellobiohydrolases. Pectinolytic activity, instead, was improved in the presence of p -coumaric acid, trans-ferulic acid, and syringaldehyde.