The role of catalase in assimilation of exogenous methanol by Chlamydomonas reinhardtii cells

Cultivation in liquid Kessler’s medium containing 0.2 % methanol stimulated the growth of Chlamydomonas reinhardtii autotrophic batch culture. To elucidate the mechanism, we examined the effects of methanol on the enzymatic activity of catalase, catalase gene (CAT1) expression, and ultrastructure of...

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Bibliographic Details
Published in:Journal of applied phycology 2020-04, Vol.32 (2), p.1053-1062
Main Authors: Stepanov, Serhiy S., Zolotareva, Elena K., Belyavskaya, Ninel A.
Format: Article
Language:English
Subjects:
Batch culture
Biomedical and Life Sciences
Catalase
Cell culture
Cells
Chemical evolution
Chlamydomonas reinhardtii
Chloroplasts
Cultivation
DNA
Ecology
Electron microscopy
Enzymatic activity
Enzyme activity
Freshwater & Marine Ecology
Gene expression
Hydrogen peroxide
Life Sciences
Localization
Methanol
Mitochondria
Nucleotide sequence
Oxidation
PCR
Plant Physiology
Plant Sciences
Polymerase chain reaction
Transmission electron microscopy
Ultrastructure
Vacuoles
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Summary:Cultivation in liquid Kessler’s medium containing 0.2 % methanol stimulated the growth of Chlamydomonas reinhardtii autotrophic batch culture. To elucidate the mechanism, we examined the effects of methanol on the enzymatic activity of catalase, catalase gene (CAT1) expression, and ultrastructure of C. reinhardtii cells. CAT1 relative expression was detected by real time RT-PCR. The cellular ultrastructure was investigated using transmission electron microscopy (TEM). Catalase-mediated oxygen evolution from H 2 O 2 was assayed with a Clark-type electrode. The localization of catalase activity in C. reinhardtii cells grown in the presence of methanol was studied by a method based on cytochemical staining with 3,3'-diaminobenzidine-tetrahydrochloride (DAB). The cytochemical data obtained in this study confirmed catalase localization in mitochondria of C. reinhardtii cells. It was shown biochemically that mitochondrial catalase activity of the alga can be enhanced by methanol. CAT1 gene expression after 4 h of growth with methanol was 16-fold higher than in the control samples. Methanol addition induced quantitative changes in ultrastructure of C. reinhardtii cells: the volume of C. reinhardtii cells and fractions of cell area occupied by chloroplasts decreased, the areas of vacuoles, mitochondria, and plastoglobules increased. The results suggest that mitochondrial catalase takes part in the oxidation of methanol.
ISSN:0921-8971
1573-5176
DOI:10.1007/s10811-019-01962-y