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Differential molecular responses of aphid-sensitive and aphid-resistant sorghum lines to aphid infestation

We used the cDNA-AFLP method to analyze gene expression changes of two sorghum lines with different resistance to aphids during aphid infestation. We identified 65 differential display fragments, including 6 sequences that were different between resistant and susceptible mRNA pools without aphid ind...

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Bibliographic Details
Published in:Arthropod-plant interactions 2012-03, Vol.6 (1), p.113-120
Main Authors: Chang, Jinhua, Duan, Xifei, Cui, Jianghui, Xue, Wei, Zhang, Qingwen
Format: Article
Language:English
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Summary:We used the cDNA-AFLP method to analyze gene expression changes of two sorghum lines with different resistance to aphids during aphid infestation. We identified 65 differential display fragments, including 6 sequences that were different between resistant and susceptible mRNA pools without aphid induction. The amplified fragments were sequenced to identify the corresponding genes. A total of 23 sequences were identified. This included 2 sequences (sequences 1 and 4) that are expressed ubiquitously in the aphid-resistant cultivar but are only expressed during infestation of the sensitive cultivar. Sequence searches by BLASTx and BLASTn software showed that sequence 1 was 97% (194/199) similar to the AOS gene from Oryza sativa and that sequence 4 was 98% (298/304) similar to the LOX-2 mRNA from Oryza sativa. Other sequences were divided into 9 functional classes involved in plant resistance to disease and insect infestation. The expression of sequence 4 was evaluated by real-time PCR. The results show that the transcript of sequence 4 was induced by aphid and the expression was upregulated, but the amount of sequence 4 transcript in resistant plants was higher than in susceptible plants.
ISSN:1872-8855
1872-8847
DOI:10.1007/s11829-011-9159-y