Evaluation of cholesterol absorption inhibition of Anti-NPC1L1 IgY by [3H]-labeled cholesterol in Sprague-Dawley rats

This research was conducted to assess the ability of [ 3 H]-cholesterol by co-administered Anti-NPC1L1 (Niemann-Pick C1-Like 1) IgY for measuring the absorption of cholesterol and to evaluate the efficacy of Anti-NPC1L1 IgY as a cholesterol absorption inhibitor in Sprague-Dawley rats model. The rats...

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Bibliographic Details
Published in:Journal of radioanalytical and nuclear chemistry 2015-07, Vol.305 (1), p.315-321
Main Authors: Utami, Hayu Tyas, Cho, Jae-Young, Jang, Beom-Su, Park, Sang-Hyun
Format: Article
Language:English
Subjects:
Absorption
Antilipemic agents
Bile
Chemistry
Chemistry and Materials Science
Cholesterol
Diagnostic Radiology
Evaluation
Hadrons
Heavy Ions
Inorganic Chemistry
Intestine
Nuclear Chemistry
Nuclear Physics
Physical Chemistry
Radioactivity
Rodents
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Summary:This research was conducted to assess the ability of [ 3 H]-cholesterol by co-administered Anti-NPC1L1 (Niemann-Pick C1-Like 1) IgY for measuring the absorption of cholesterol and to evaluate the efficacy of Anti-NPC1L1 IgY as a cholesterol absorption inhibitor in Sprague-Dawley rats model. The rats were administered with Anti-NPC1L1 IgY, ezetimibe as positive control and yolk powder as vehicle control. Systemic blood, feces, urine and intestine were sampled and the concentration of cholesterol was determined by means of radioactivity. The results indicate that the evaluation of cholesterol absorption inhibition was successfully performed by [ 3 H]-cholesterol. Meanwhile, the administration of Anti-NPC1L1 IgY inhibits cholesterol absorption in Sprague-Dawley rats significantly.
ISSN:0236-5731
1588-2780
DOI:10.1007/s10967-015-3996-6