Reference genes selection for real-time quantitative PCR analysis in mouse germinal vesicle oocytes

Reference gene selection in mouse oocytes is an important task required to perform further adequate analysis of target gene expression levels. In the current work we have analyzed expression stability of the seven most commonly used reference genes (Actb, Eef1e1, Gapdh, H2afz, Ppia, Rpl4 and Ubc) in...

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Bibliographic Details
Published in:Zygote (Cambridge) 2019-12, Vol.27 (6), p.392-397
Main Authors: Filatov, M.A., Nikishin, D.A., Khramova, Y.V., Semenova, M.L.
Format: Article
Language:English
Subjects:
Animals
Female
Gametocytes
Gene expression
Gene Expression Profiling - methods
Gene Expression Regulation, Developmental
Genes
Glyceraldehyde-3-phosphate dehydrogenase
Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating) - genetics
Mice
Oocytes
Oocytes - cytology
Oocytes - metabolism
Oogenesis - genetics
Peptide Elongation Factors - toxicity
Real-Time Polymerase Chain Reaction - methods
Reference Standards
Ribosomal Proteins - genetics
Software
Software development tools
Stability analysis
Studies
Tumor Suppressor Proteins - toxicity
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Summary:Reference gene selection in mouse oocytes is an important task required to perform further adequate analysis of target gene expression levels. In the current work we have analyzed expression stability of the seven most commonly used reference genes (Actb, Eef1e1, Gapdh, H2afz, Ppia, Rpl4 and Ubc) in mouse oocytes at the germinal vesicle (GV) stage. We have performed analysis of expression stability of the above-mentioned reference genes with the three most commonly used software tools: geNorm, BestKeeper and NormFinder. Taking into account the results obtained from all of these programmes Gapdh, Rpl4 and H2afz seem to be suitable candidate reference genes in GV oocytes of mouse.
ISSN:0967-1994
1469-8730
DOI:10.1017/S0967199419000492