Purification and relationship with gastric disease of a 130 kDa (CagA) protein ofHelicobacter pylori

Objective: The aims of this research were to purify and identify the 130 kDa (CagA) protein ofH. pylori clinical isolate HP97002 and evaluate the relationships between the purified 130 kDa (CagA) protein and gastric diseases. Methods: The procedure for isolating the protein included 6 mol/L guanidin...

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Published in:Journal of Zhejiang University. A. Science 2003-03, Vol.4 (2), p.232-235
Main Authors: Ye, Shao-jing, Fang, Ping-chu, Mao, Guo-gen, Li, Chao-long, Qiu, Xiang, Chen, Hai-xiang
Format: Article
Language:English
Subjects:
Antigenicity
Cancer
Disease
Diseases
Gastric cancer
Gastritis
Guanidine
Health risk assessment
Peptic ulcers
Protein purification
Proteins
Size exclusion chromatography
Ulcers
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Summary:Objective: The aims of this research were to purify and identify the 130 kDa (CagA) protein ofH. pylori clinical isolate HP97002 and evaluate the relationships between the purified 130 kDa (CagA) protein and gastric diseases. Methods: The procedure for isolating the protein included 6 mol/L guanidine extract, size exclusion chromatography and elusion from gel. Sera of 68 patients with gastric diseases (44 with chronic gastritis, 15 with atrophic gastritis, 7 with peptic ulcer disease, 2 with gastric cancer) were obtained, and the serological response to CagA was studied by Western-blot using the purified protein. Results: The purified protein was 130 kDa and preserved good antigenicity and revealed basic isoelectric point about of 8.1. Among 68 sera, 43 sera could recognize the purified protein associated with chronic gastritis 47.7% (21/44), atrophic gastritis 86.7% (13/15), peptic ulcer disease 100% (7/7), gastric cancer 100% (2/2). Compared with each other, the difference was significant (χ2=13.327,P=0.004), and 130 kDa (CagA) protein was associated with severe gastric diseases (rs=0.442,P=0.001). Conclusion: The 130 kDa (CagA) protein was associated with severe gastric diseases.
ISSN:1673-565X
1862-1775
DOI:10.1631/jzus.2003.0232