Isolation of Fluoride Tolerant Bacillus spp (KT201599, KT201600) from the Midgut of Drosophila melanogaster: Their Probable Role in Fluoride Removal

This is for the first time that two fluoride tolerant bacterial strains of Bacillus spp (KT201599 and KT201600) have been isolated from the mid-gut of third instar larvae of Drosophila melanogaster. They are characterized and identified using phenotypic characters and 16S rRNA gene based molecular p...

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Bibliographic Details
Published in:Proceedings of the Zoological Society 2020-06, Vol.73 (2), p.175-183
Main Authors: Dutta, Moumita, Pan, Bappaditya, Ghosh, Koushik, Saha, Pradipta, Roy, Sumedha
Format: Article
Language:English
Subjects:
Animal Anatomy
Animal Genetics and Genomics
Bacillus
Biodiversity
Biomedical and Life Sciences
Conservation Biology/Ecology
Drosophila
Drosophila melanogaster
Fluorides
Histology
Insects
Intestinal microflora
Life Sciences
Midgut
Morphology
Phylogeny
Research Article
rRNA 16S
Sodium fluoride
Strains (organisms)
Zoology
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Summary:This is for the first time that two fluoride tolerant bacterial strains of Bacillus spp (KT201599 and KT201600) have been isolated from the mid-gut of third instar larvae of Drosophila melanogaster. They are characterized and identified using phenotypic characters and 16S rRNA gene based molecular phylogenetic analysis. Interestingly, KT201599 and KT201600 are able to survive at 2000–2500 µg/mL sodium fluoride (NaF) concentrations. The chronic lethal concentration 50 (LC 50 ) for NaF in Drosophila larvae is 125 µg/mL, whereas, the above-mentioned Bacillus strains isolated from their mid-gut are found to be more tolerant to fluoride (F) in comparison to their host. In search for biological relevance of KT201599 and KT201600, we further deciphered that these strains not only survived at high F concentrations, but also were efficient in removing the fluoride ions from the medium by 16.66 and 24.71% respectively. Thus, the study indicates that resident gut flora of Drosophila having the property of reducing fluoride load from the medium, might be helping the host to thrive in a fluoridated environment.
ISSN:0373-5893
0974-6919
DOI:10.1007/s12595-018-0282-y