Multivalent cationic dendrofullerenes for gene transfer: synthesis and DNA complexation

Non-viral nucleic acid vectors able to display high transfection efficiencies with low toxicity and overcoming the multiple biological barriers are needed to further develop the clinical applications of gene therapy. The synthesis of hexakis-adducts of [60]fullerene endowed with 12, 24 and 36 positi...

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Bibliographic Details
Published in:Journal of materials chemistry. B, Materials for biology and medicine Materials for biology and medicine, 2020-05, Vol.8 (2), p.455-4515
Main Authors: Illescas, Beatriz M, Pérez-Sánchez, Alfonso, Mallo, Araceli, Martín-Domenech, Ángel, Rodríguez-Crespo, Ignacio, Martín, Nazario
Format: Article
Language:English
Subjects:
Adducts
Ammonium
Cations - chemical synthesis
Cations - chemistry
Complexation
Cytotoxicity
Dendrimers - chemical synthesis
Dendrimers - chemistry
Deoxyribonucleic acid
DNA
DNA - chemistry
DNA biosynthesis
Fluorescence
Fullerenes
Fullerenes - chemistry
Gene expression
Gene therapy
Gene Transfer Techniques
Green fluorescent protein
Green Fluorescent Proteins - genetics
HEK293 Cells
Humans
Macromolecules
Molecular Structure
NMR
Nuclear magnetic resonance
Nucleic acids
Particle Size
Surface Properties
Synthesis
Therapeutic applications
Toxicity
Transfection
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Summary:Non-viral nucleic acid vectors able to display high transfection efficiencies with low toxicity and overcoming the multiple biological barriers are needed to further develop the clinical applications of gene therapy. The synthesis of hexakis-adducts of [60]fullerene endowed with 12, 24 and 36 positive ammonium groups and a tridecafullerene appended with 120 positive charges has been performed. The delivery of a plasmid containing the green fluorescent protein (EGFP) gene into HEK293 (Human Embryonic Kidney) cells resulting in effective gene expression has demonstrated the efficacy of these compounds to form polyplexes with DNA. Particularly, giant tridecafullerene macromolecules have shown higher efficiency in the complexation and transfection of DNA. Thus, they can be considered as promising non-viral vectors for transfection purposes. Non-viral nucleic acid vectors able to display high transfection efficiencies with low toxicity and overcoming the multiple biological barriers are needed to further develop the clinical applications of gene therapy.
ISSN:2050-750X
2050-7518
DOI:10.1039/d0tb00113a