A reliable method for individual identification and gender determination of wild leopards (Panthera pardus fusca) using non-invasive samples

We describe a highly polymorphic microsatellite panel for identifying individual leopards using DNA from scat. After successfully screening 16 published microsatellites, we optimized a panel of 7 microsatellites that yields a Probability of Identity between siblings value of 5.24E−04. We used this p...

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Bibliographic Details
Published in:Conservation genetics resources 2012-09, Vol.4 (3), p.665-667
Main Authors: Dutta, Trishna, Sharma, Sandeep, Maldonado, Jesús E., Wood, Thomas C., Seidensticker, John
Format: Article
Language:English
Subjects:
Animal Genetics and Genomics
Biodiversity
Biomedical and Life Sciences
Conservation Biology/Ecology
Ecology
Evolutionary Biology
Gene flow
Genetic structure
Life Sciences
Microsatellites
Plant Genetics and Genomics
Sex ratio
Technical Note
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Summary:We describe a highly polymorphic microsatellite panel for identifying individual leopards using DNA from scat. After successfully screening 16 published microsatellites, we optimized a panel of 7 microsatellites that yields a Probability of Identity between siblings value of 5.24E−04. We used this panel to identify 217 individuals from 287 leopard scats collected from five tiger-reserves in Central India. We identified 101 males and 92 females by amplifying a fragment of the Amelogenin protein gene. This panel will be helpful to study genetic structure, gene flow, relatedness and sex ratio of leopards.
ISSN:1877-7252
1877-7260
DOI:10.1007/s12686-012-9618-5