Development and characterization of thirty-three microsatellite markers for the Patagonian sprat, Sprattus fuegensis (Jenyns, 1842), using paired-end Illumina shotgun sequencing

We isolated and characterized a total of 33 microsatellite loci from the Patagonian sprat Sprattus fuegensis, a recent exploited marine resource with a conservation status unknowing. Loci were screened in 24 individuals from the inshore waters of the Aysén Fjord, Chile. The number of alleles per loc...

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Bibliographic Details
Published in:Conservation genetics resources 2014-12, Vol.6 (4), p.833-836
Main Authors: Ferrada-Fuentes, Sandra, Galleguillos, Ricardo, Canales-Aguirre, Cristian B, Love, Cara N, Jones, Kenneth L, Lance, Stacey L
Format: Article
Language:English
Subjects:
alleles
Animal Genetics and Genomics
Biodiversity
Biomedical and Life Sciences
Conservation
Conservation Biology/Ecology
Conservation status
Ecology
Evolutionary Biology
fisheries management
Fishery management
Fjords
Genetic markers
Genetic structure
Heterozygosity
Life Sciences
loci
marine resources
microsatellite repeats
Microsatellites
Plant Genetics and Genomics
Population genetics
population size
probability
Sprattus
Sprattus sprattus
Technical Note
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Summary:We isolated and characterized a total of 33 microsatellite loci from the Patagonian sprat Sprattus fuegensis, a recent exploited marine resource with a conservation status unknowing. Loci were screened in 24 individuals from the inshore waters of the Aysén Fjord, Chile. The number of alleles per locus ranged from 7 to 24, observed heterozygosity ranged from 0.217 to 0.875, and the probability of identity values ranged from 0.006 to 0.133. These new loci will provide tools for examining population genetic structure, estimating effective population size and provide information to fisheries management and conservation.
ISSN:1877-7252
1877-7260
DOI:10.1007/s12686-014-0281-x