Isolation and characterization of twelve polymorphic microsatellite loci for investigating an extreme case of microgeographical variation in an island bird (Zosterops borbonicus)

Twelve polymorphic microsatellite loci were characterized in order to investigate an extreme case of microgeographical variation in an island-dwelling passerine ( Zosterops borbonicus ). Variation was examined in two distinct natural populations of 27 individuals each. The loci displayed 5–20 allele...

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Bibliographic Details
Published in:Conservation genetics resources 2012-06, Vol.4 (2), p.323-326
Main Authors: Bertrand, Joris A. M., García-Jiménez, Ricardo, Bourgeois, Yann, Duval, Thomas, Heeb, Philipp, Thébaud, Christophe, Milá, Borja
Format: Article
Language:English
Subjects:
Animal Genetics and Genomics
Biodiversity
Biomedical and Life Sciences
Conservation Biology/Ecology
Ecology
Evolutionary Biology
Life Sciences
Linkage disequilibrium
Plant Genetics and Genomics
Population genetics
Technical Note
Variation
Zosterops borbonicus
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Summary:Twelve polymorphic microsatellite loci were characterized in order to investigate an extreme case of microgeographical variation in an island-dwelling passerine ( Zosterops borbonicus ). Variation was examined in two distinct natural populations of 27 individuals each. The loci displayed 5–20 alleles, with observed heterozygosities ranging between 0.33 and 0.93. All but one loci (Z16) conformed to Hardy–Weinberg equilibrium. None of the pairwise comparisons among loci showed significant linkage disequilibrium after sequential Bonferonni correction. Successful cross-species amplification in additional Zosterops species and other passerines suggests their broad applicability.
ISSN:1877-7252
1877-7260
DOI:10.1007/s12686-011-9538-9