Development and characterization of ten microsatellite markers for population studies of the native Brazilian oyster Crassostrea gasar

• Published in: Conservation genetics resources 2012-09, Vol.4 (3), p.583-586
• Main Authors: Melo, Mauro André Damasceno, da Silva, Adam Rick Bessa, Varela, Eduardo Sousa, Sampaio, Iracilda, Tagliaro, Claudia Helena
• Format: Article
• Language: English
• Subjects: Animal Genetics and Genomics; Biodiversity; Biomedical and Life Sciences; Conservation Biology/Ecology; Crassostrea gasar; Ecology; Evolutionary Biology; Genetic markers; Heterozygosity; Indigenous species; Life Sciences; Microsatellites; Plant Genetics and Genomics; Population genetics; Population studies; Technical Note
• ISSN: 1877-7252; 1877-7260
• DOI: 10.1007/s12686-011-9597-y

A set of ten polymorphic microsatellite loci were isolated and characterized from a CT/GT enriched genomic library of the Brazilian native oyster Crassostrea gasar (Adanson, 1757). These markers were polymorphic and successfully amplified in 21 individuals from São João de Pirabas, Pará state, Brazil. The number of alleles per polymorphic locus varied from 6 to 18 and values of observed and expected heterozygosity ranged from 0.3333 to 1.0000 and from 0.6655 to 0.9361, respectively. Only one of the ten microsatellite loci exhibited deviation from Hardy–Weinberg equilibrium and no evidence of linkage was detected between loci. These informative genetic markers will be very useful in population genetic studies of the mangrove oyster C . gasar . The ten markers also showed cross-species transferability with the native oyster Crassostrea rhizophorae .