Characterization of nine microsatellite loci from the endangered southern brown bandicoot (Isoodon obesulus) using 454 pyrosequencing

Nine polymorphic microsatellite markers were developed for a nationally endangered marsupial, the southern brown bandicoot ( Isoodon obesulus ) using a next generation sequencing approach. The nine markers were genotyped in 59 individuals from two distinct locations (the Mt Burr Range and the Mt Lof...

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Bibliographic Details
Published in:Conservation genetics resources 2013-03, Vol.5 (1), p.105-107
Main Authors: Li, You, Lancaster, Melanie L., Cooper, Steven J. B., Packer, Jasmin G., Carthew, Susan M.
Format: Article
Language:English
Subjects:
Animal Genetics and Genomics
Biodiversity
Biomedical and Life Sciences
Conservation Biology/Ecology
Conservation genetics
Ecology
Evolutionary Biology
Genetic markers
Isoodon obesulus
Life Sciences
Linkage disequilibrium
Microsatellites
Next-generation sequencing
Plant Genetics and Genomics
Population genetics
Technical Note
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Summary:Nine polymorphic microsatellite markers were developed for a nationally endangered marsupial, the southern brown bandicoot ( Isoodon obesulus ) using a next generation sequencing approach. The nine markers were genotyped in 59 individuals from two distinct locations (the Mt Burr Range and the Mt Lofty Ranges) in South Australia. All loci showed Hardy–Weinberg equilibrium with the exception of one locus in the Mt Lofty population, possibly because of null alleles. No evidence of linkage disequilibrium was detected. These markers will provide valuable resources for future projects on the conservation genetics of southern brown bandicoots in Australia.
ISSN:1877-7252
1877-7260
DOI:10.1007/s12686-012-9743-1