Development of 20 TaqMan assays differentiating the endangered shortnose and Lost River suckers

Accurate species identification is vital to conservation and management of species at risk. Species identification is challenging when taxa express similar phenotypic characters and form hybrids, for example the endangered shortnose sucker ( Chasmistes brevirostris ) and Lost River sucker ( Deltiste...

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Bibliographic Details
Published in:Conservation genetics resources 2015-09, Vol.7 (3), p.673-676
Main Authors: Hoy, M. S., Ostberg, C. O.
Format: Article
Language:English
Subjects:
Animal Genetics and Genomics
Biodiversity
Biomedical and Life Sciences
Chasmistes brevirostris
Conservation Biology/Ecology
Deltistes luxatus
Deoxyribonucleic acid
DNA
Ecology
Endangered species
Evolutionary Biology
Hybrids
Life Sciences
Mitochondrial DNA
Plant Genetics and Genomics
Rivers
Technical Note
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Summary:Accurate species identification is vital to conservation and management of species at risk. Species identification is challenging when taxa express similar phenotypic characters and form hybrids, for example the endangered shortnose sucker ( Chasmistes brevirostris ) and Lost River sucker ( Deltistes luxatus ). Here, we developed 20 Taqman assays that differentiate these species (19 nuclear DNA and one mitochondrial DNA). Assays were evaluated in 160 young-of-the-year identified to species using meristic counts. Alleles were not fixed between species, but species were highly differentiated ( F ST  = 0.753, P  
ISSN:1877-7252
1877-7260
DOI:10.1007/s12686-015-0474-y