Microsatellite loci in the oceanic whitetip shark and cross-species amplification using pyrosequencing technology

Among the pelagic shark species most impacted by fisheries, the oceanic whitetip shark ( Carcharhinus longimanus ) has shown signs of population declines. In this study, we developed 12 microsatellite markers using next generation sequencing (454 pyrosequencing) in C. longimanus for further use in p...

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Bibliographic Details
Published in:Conservation genetics resources 2015-06, Vol.7 (2), p.585-589
Main Authors: Mendes, N. J., Cruz, V. P., Mendonça, Fernando Fernandes, Pardo, B. G., Coelho, R., Ashikaga, F. Y., Camargo, S. M., Martínez, P., Oliveira, C., Santos, M. N., Foresti, F.
Format: Article
Language:English
Subjects:
Alleles
Animal Genetics and Genomics
Biodiversity
Biomedical and Life Sciences
Carcharhinus longimanus
Conservation Biology/Ecology
Ecology
Evolutionary Biology
Galeocerdo cuvier
Genetic diversity
Genetic markers
Life Sciences
Microsatellite Letters
Microsatellites
Next-generation sequencing
Plant Genetics and Genomics
Population decline
Population genetics
Population studies
Sharks
Species
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Summary:Among the pelagic shark species most impacted by fisheries, the oceanic whitetip shark ( Carcharhinus longimanus ) has shown signs of population declines. In this study, we developed 12 microsatellite markers using next generation sequencing (454 pyrosequencing) in C. longimanus for further use in population screening. The genetic diversity of these loci was assessed in 28 individuals and a total of 35 alleles were identified, ranging from four to eight alleles per locus (5.9 alleles on average). Cross-species amplification of these markers was successful in five loci for C. acronotus , C. perezi and Galeocerdo cuvier . The developed microsatellite markers will be useful in analyzing the population genetics structure of the oceanic whitetip shark and other Carcharhinidae species.
ISSN:1877-7252
1877-7260
DOI:10.1007/s12686-015-0435-5