A rapid method for species identification and interspecies introgression based on a PCR–RFLP: application to the closely related Neotropical toads Rhinella marina and R. schneideri (Anura: Bufonidae)

Toad species Rhinella marina and R. schneideri (Bufonidae, Anura) are widely distributed in Amazon and Cerrado/Caatinga, respectively. Recent data has hypothesized that these two toad species share a history of a massive introgressive hybridization. Therefore, the aim of the present work was the pre...

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Published in:Conservation genetics resources 2013-03, Vol.5 (1), p.227-229
Main Authors: Cunha, D. B., Sodré, D., Santos, A. C. A., Schneider, H., Sampaio, I., Sequeira, F., Vallinoto, M.
Format: Article
Language:English
Subjects:
Animal Genetics and Genomics
Anura
Biodiversity
Biomedical and Life Sciences
Bufonidae
Conservation Biology/Ecology
Ecology
Evolutionary Biology
Gene polymorphism
Hybridization
Hybrids
Life Sciences
Plant Genetics and Genomics
Polymerase chain reaction
Restriction fragment length polymorphism
Rhinella marina
Rhinella schneideri
Species
Technical Note
Toads
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cited_by cdi_FETCH-LOGICAL-c316t-d3d01e36c08a1ac822481ae2c2e121f829212465a66a0525250b9301b44557b23
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container_start_page 227
container_title Conservation genetics resources
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creator Cunha, D. B.
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description Toad species Rhinella marina and R. schneideri (Bufonidae, Anura) are widely distributed in Amazon and Cerrado/Caatinga, respectively. Recent data has hypothesized that these two toad species share a history of a massive introgressive hybridization. Therefore, the aim of the present work was the precise identification of organisms with signs of past introgression or actual ongoing hybridization. In this paper we conducted a method based on polymerase chain reaction–restriction fragment length polymorphism, through the use of the CfoI enzyme. The individuals of R. marina have two specific digested bands, which is not digested in R. schneideri , possessing the hybrids three bands. The method was very efficient and allowed fast identification of these species.
doi_str_mv 10.1007/s12686-012-9775-6
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source Springer Nature
subjects Animal Genetics and Genomics
Anura
Biodiversity
Biomedical and Life Sciences
Bufonidae
Conservation Biology/Ecology
Ecology
Evolutionary Biology
Gene polymorphism
Hybridization
Hybrids
Life Sciences
Plant Genetics and Genomics
Polymerase chain reaction
Restriction fragment length polymorphism
Rhinella marina
Rhinella schneideri
Species
Technical Note
Toads
title A rapid method for species identification and interspecies introgression based on a PCR–RFLP: application to the closely related Neotropical toads Rhinella marina and R. schneideri (Anura: Bufonidae)
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