Isolation and characterization of 15 tetranucleotide microsatellite loci in the desert iguana (Dipsosaurus dorsalis, Baird and Girard) by 454 pyrosequencing

New microsatellite loci were developed for the Desert iguana ( Dipsosaurus dorsalis ) through GS-FLX 454 pyrosequencing. We selected 15 primer pairs that were tested on 23 individuals from San Isidro area in the southern part of Baja California Peninsula. We observed low to medium genetic variation...

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Bibliographic Details
Published in:Conservation genetics resources 2013-09, Vol.5 (3), p.719-721
Main Authors: Valdivia Carrillo, Tania, Lozano Garza, O. Adrián, García de León, Francisco J., Blázquez, María Carmen
Format: Article
Language:English
Subjects:
Animal Genetics and Genomics
Biodiversity
Biomedical and Life Sciences
Conservation Biology/Ecology
Dipsosaurus dorsalis
Ecology
Evolutionary Biology
Genetic diversity
Life Sciences
Plant Genetics and Genomics
Population studies
Technical Note
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Summary:New microsatellite loci were developed for the Desert iguana ( Dipsosaurus dorsalis ) through GS-FLX 454 pyrosequencing. We selected 15 primer pairs that were tested on 23 individuals from San Isidro area in the southern part of Baja California Peninsula. We observed low to medium genetic variation among loci (mean number of alleles per locus = 7, mean H O  = 0.696). These loci are the first characterized for D. dorsalis and they are expected to be useful for population level studies in this species.
ISSN:1877-7252
1877-7260
DOI:10.1007/s12686-013-9890-z