A highly informative microsatellite panel for individual identification and sex determination of jungle cats (Felis chaus)

The jungle cat ( Felis chaus ), a small-sized felid, is distributed across Southeast and South Asia to Egypt in Africa. In India, it is one of the most common small cat species but no reliable methods are available to monitor its population status. We describe a non-invasive genetic monitoring techn...

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Bibliographic Details
Published in:Conservation genetics resources 2013-09, Vol.5 (3), p.863-866
Main Authors: Sharma, Sandeep, Dutta, Trishna, Maldonado, Jesús E., Wood, Thomas C., Panwar, Hemendra Singh, Seidensticker, John
Format: Article
Language:English
Subjects:
Animal Genetics and Genomics
Application Essays
Biodiversity
Biomedical and Life Sciences
Conservation Biology/Ecology
Ecology
Evolutionary Biology
Felis chaus
Gene flow
Genetic structure
Life Sciences
Microsatellites
Plant Genetics and Genomics
Population status
Sex determination
Sex ratio
Wildcats
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Summary:The jungle cat ( Felis chaus ), a small-sized felid, is distributed across Southeast and South Asia to Egypt in Africa. In India, it is one of the most common small cat species but no reliable methods are available to monitor its population status. We describe a non-invasive genetic monitoring technique using fecal samples for individual identification and sex determination of jungle cats. We evaluated 21 feline microsatellites and optimized a panel of 11 highly polymorphic microsatellite loci that yield a cumulative Probability of Identity between siblings value of 5.51×10 −6 . We used this panel to identify 110 individuals from 118 jungle cat scats collected from tiger reserves in Central India. We identified 22 males and 35 females by amplifying a fragment of the Amelogenin protein gene. This panel will be helpful to study genetic structure, gene flow, relatedness, sex ratio, and population estimation in jungle cats.
ISSN:1877-7252
1877-7260
DOI:10.1007/s12686-013-9873-0