Isolation and characterization of twelve novel polymorphic microsatellite loci in the Branchiostoma belcheri Gray (Amphioxus)

Branchiostoma belcheri Gray is an endangered species. A set of twelve novel polymorphic microsatellite loci was developed in B. belcheri Gray. The polymorphism of these loci was tested on a population of thirty individuals from Xiamen, China. The number of alleles ranged from 3 to 7. The polymorphis...

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Bibliographic Details
Published in:Conservation genetics resources 2013-12, Vol.5 (4), p.1115-1116
Main Authors: Dai, Gang, Li, Yuanyue, Li, Zhongbao, Li, Qinghui, Cao, Yuanyu, Shangguan, Jingbo, Ning, Yefeng, Yang, Huan, Lu, Jing
Format: Article
Language:English
Subjects:
alleles
Animal Genetics and Genomics
Biodiversity
Biomedical and Life Sciences
Branchiostoma belcheri
China
Conservation Biology/Ecology
Ecology
Endangered species
Evolutionary Biology
Life Sciences
loci
Microsatellite Letters
microsatellite repeats
Plant Genetics and Genomics
Polymorphism
Population genetics
Population studies
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Summary:Branchiostoma belcheri Gray is an endangered species. A set of twelve novel polymorphic microsatellite loci was developed in B. belcheri Gray. The polymorphism of these loci was tested on a population of thirty individuals from Xiamen, China. The number of alleles ranged from 3 to 7. The polymorphism information content ranged from 0.309 to 0.778. The levels of expected and observed heterozygosities varied from 0.2449 to 0.7825, and from 0.1786 to 0.8571, respectively. Three loci (WCY4-22, WCY4-39, WCY5-17) deviated from Hardy–Weinberg equilibrium. These microsatellite loci would contribute to population genetic studies and conservation of B. belcheri Gray.
ISSN:1877-7252
1877-7260
DOI:10.1007/s12686-013-9966-9