Isolation and characterization of 29 polymorphic microsatellite markers for the endangered Atlantic goliath grouper (Epinephelus itajara), and the Pacific goliath grouper (E. quinquefasciatus)

We isolated 40 microsatellite markers for the endangered Atlantic, and Pacific goliath grouper, Epinephelus itajara and E. quinquefasciatus . Loci were characterized using samples of E. itajara ( n  = 52) and E. quinquefasciatus ( n  = 10). Twenty-nine loci were polymorphic. For E. itajara and E. qu...

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Bibliographic Details
Published in:Conservation genetics resources 2013-09, Vol.5 (3), p.729-732
Main Authors: Seyoum, Seifu, Tringali, Michael D., Barthel, Brandon L., Puchulutegui, Cecilia, Davis, Michelle C., Collins, Angela B., Craig, Matthew T.
Format: Article
Language:English
Subjects:
Alleles
Animal Genetics and Genomics
Biodiversity
Biomedical and Life Sciences
Conservation Biology/Ecology
Ecology
Epinephelus itajara
Evolutionary Biology
Gene frequency
Genetic markers
Life Sciences
Microsatellites
Plant Genetics and Genomics
Technical Note
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Summary:We isolated 40 microsatellite markers for the endangered Atlantic, and Pacific goliath grouper, Epinephelus itajara and E. quinquefasciatus . Loci were characterized using samples of E. itajara ( n  = 52) and E. quinquefasciatus ( n  = 10). Twenty-nine loci were polymorphic. For E. itajara and E. quinquefasciatus , respectively, mean numbers of alleles per locus were 5.2 and 4.3 and mean observed heterozygosities were 0.46 and 0.63. Three loci departed from Hardy–Weinberg equilibrium expectations in the E. itajara sample. Allele frequencies differed significantly between the two taxa for each polymorphic locus.
ISSN:1877-7252
1877-7260
DOI:10.1007/s12686-013-9892-x