Development of 24 tetra-nucleotide microsatellite markers in Cortes Geoduck Panopea globosa by next-generation sequencing

Panopea globosa is a marine bivalve mollusk important for small-scale fisheries from Northwest Mexico. Twenty-four tetra-nucleotide microsatellite markers were characterized by 454 XL+ Titanium pyrosequencing. High average levels of allelic diversity and heterozygosity were observed (N A = 16.68; Ne...

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Bibliographic Details
Published in:Conservation genetics resources 2014-09, Vol.6 (3), p.531-533
Main Authors: Cruz-Hernández, Pedro, Munguia-Vega, Adrian, Leyva-Valencia, Ignacio, Lucero-Burquez, Fátima, Lluch-Cota, Daniel B
Format: Article
Language:English
Subjects:
Animal Genetics and Genomics
Biodiversity
Biomedical and Life Sciences
Conservation Biology/Ecology
Ecology
Evolutionary Biology
fisheries
Genetic diversity
Genetic markers
Genetic structure
Heterozygosity
high-throughput nucleotide sequencing
larvae
Life Sciences
Linkage disequilibrium
loci
microsatellite repeats
Microsatellites
molluscs
Next-generation sequencing
Panopea
Panopea globosa
Plant Genetics and Genomics
Technical Note
Titanium
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Summary:Panopea globosa is a marine bivalve mollusk important for small-scale fisheries from Northwest Mexico. Twenty-four tetra-nucleotide microsatellite markers were characterized by 454 XL+ Titanium pyrosequencing. High average levels of allelic diversity and heterozygosity were observed (N A = 16.68; Ne = 9.77; H O = 0.643; H E = 0.858). Significant deviations from HWE, as previously observed in other mollusks, were detected in twelve loci. Linkage disequilibrium was detected among two loci pairs. This set of microsatellite loci will be useful to determine the genetic structure and larval connectivity of P. globosa.
ISSN:1877-7252
1877-7260
DOI:10.1007/s12686-014-0172-1