Development and multiplex genotyping of eighteen novel microsatellite markers for a threatened saltmarsh bird Epthianura albifrons (Meliphagidae)

Using 454 pyrosequencing and multiplex genotyping we developed 18 novel microsatellite loci in Epthianura albifrons (Jardine & Selby, 1828), a small passerine threatened by loss of coastal saltmarsh. We also tested the utility of 21 previously-developed microsatellite loci from two con-familial...

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Bibliographic Details
Published in:Conservation genetics resources 2012-12, Vol.4 (4), p.893-896
Main Authors: King, Andrew G., Cooke, Georgina M., Johnson, Rebecca N., Major, Richard E.
Format: Article
Language:English
Subjects:
Animal Genetics and Genomics
Biodiversity
Biomedical and Life Sciences
Conservation Biology/Ecology
Ecology
Epthianura albifrons
Evolutionary Biology
Gene flow
Genetic markers
Genotyping
Life Sciences
Microsatellites
Plant Genetics and Genomics
Technical Note
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Summary:Using 454 pyrosequencing and multiplex genotyping we developed 18 novel microsatellite loci in Epthianura albifrons (Jardine & Selby, 1828), a small passerine threatened by loss of coastal saltmarsh. We also tested the utility of 21 previously-developed microsatellite loci from two con-familial species, however these were weakly polymorphic in E. albifrons . The 18 loci developed for E. albifrons were polymorphic with a mean of 17.1 (±2.6SE) alleles per locus. The 18 new microsatellites will be useful tools for measuring gene flow in the Endangered population of E. albifrons .
ISSN:1877-7252
1877-7260
DOI:10.1007/s12686-012-9667-9