Characterization of eight polymorphic microsatellite markers for the saw-combed fishfly, Nigronia serricornis (Say) (Megaloptera: Corydalidae)

We have developed eight polymorphic microsatellite markers for Nigronia serricornis ; the first STRs developed for the order Megaloptera. These markers were developed utilizing Illumina next-generation sequencing. Of 8,629 contigs sequenced, 38 were tested for amplification and to determine allelic...

Full description

Saved in:
Bibliographic Details
Published in:Conservation genetics resources 2013-12, Vol.5 (4), p.1111-1112
Main Authors: Stevenson, Rachel Marie, Heilveil, Jeffrey S.
Format: Article
Language:English
Subjects:
Animal behavior
Animal Genetics and Genomics
Biodiversity
Biomedical and Life Sciences
Conservation Biology/Ecology
Ecology
Evolutionary Biology
Genetic markers
Life Sciences
Megaloptera
Microsatellite Letters
Microsatellites
Next-generation sequencing
Nigronia serricornis
Plant Genetics and Genomics
Short tandem repeats
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We have developed eight polymorphic microsatellite markers for Nigronia serricornis ; the first STRs developed for the order Megaloptera. These markers were developed utilizing Illumina next-generation sequencing. Of 8,629 contigs sequenced, 38 were tested for amplification and to determine allelic richness. Of those tested, eight loci were found to be polymorphic. Markers were also tested on other members of the order Megaloptera, with various degrees of successful amplification.
ISSN:1877-7252
1877-7260
DOI:10.1007/s12686-013-9954-0