Development of 16 microsatellite loci for the endangered sandhill dunnart (Sminthopsis psammophila)

Sixteen polymorphic microsatellite loci were developed using a 454 sequencing approach, and three loci, developed for Sminthopsis douglasi, were optimized for the endangered sandhill dunnart, Sminthopsis psammophila. Nineteen loci (16 S. psammophila and three S. douglasi) were tested on 38 individua...

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Bibliographic Details
Published in:Conservation genetics resources 2014-06, Vol.6 (2), p.323-326
Main Authors: McLean, Amanda L, Cooper, Steven J. B, Lancaster, Melanie L, Carthew, Susan M
Format: Article
Language:English
Subjects:
Animal Genetics and Genomics
Biodiversity
Biomedical and Life Sciences
Conservation Biology/Ecology
Ecology
Evolutionary Biology
Genetic analysis
genetic techniques and protocols
Life Sciences
Linkage disequilibrium
loci
Microsatellite Letters
microsatellite repeats
null alleles
Plant Genetics and Genomics
Sminthopsis psammophila
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Summary:Sixteen polymorphic microsatellite loci were developed using a 454 sequencing approach, and three loci, developed for Sminthopsis douglasi, were optimized for the endangered sandhill dunnart, Sminthopsis psammophila. Nineteen loci (16 S. psammophila and three S. douglasi) were tested on 38 individual S. psammophila from two populations in South Australia. Three loci deviated from Hardy–Weinberg equilibrium, possibly due to null alleles. No evidence of linkage disequilibrium was detected. We recommend a final panel of 17 loci for future genetic analyses of S. psammophila, which will aid in conservation management of this poorly understood and nationally endangered marsupial.
ISSN:1877-7252
1877-7260
DOI:10.1007/s12686-013-0084-5