Development and characterisation of 12 microsatellite markers for the New Zealand endemic scallop Pecten novaezelandiae

The endemic scallop Pecten novaezelandiae supports important fisheries in New Zealand. However, despite the ecological and economic importance of this species, limited information is known about genetic stock structure and genetic connectivity among populations. Using 454 sequencing we developed 12...

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Bibliographic Details
Published in:Conservation genetics resources 2014-06, Vol.6 (2), p.327-328
Main Authors: Silva, Catarina N. S, Gardner, Jonathan P. A
Format: Article
Language:English
Subjects:
Alleles
Animal Genetics and Genomics
Biodiversity
Biomedical and Life Sciences
Conservation Biology/Ecology
Ecology
Economic importance
Evolutionary Biology
fisheries
Genetic markers
Genetic variability
genetic variation
Life Sciences
Linkage disequilibrium
loci
Microsatellite Letters
microsatellite repeats
Microsatellites
null alleles
Pecten
Pecten novaezelandiae
Plant Genetics and Genomics
Population genetics
scallops
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Summary:The endemic scallop Pecten novaezelandiae supports important fisheries in New Zealand. However, despite the ecological and economic importance of this species, limited information is known about genetic stock structure and genetic connectivity among populations. Using 454 sequencing we developed 12 polymorphic microsatellite markers for this scallop. The number of alleles per locus ranged from 6 to 37 and no significant linkage disequilibrium was detected between locus pairs. Three loci (Pnova_01, Pnova_24 and Pnova_27) showed significant deviations from Hardy–Weinberg equilibrium, most likely because of null alleles. The new markers are currently being used to assess the levels of genetic variability among populations to contribute to conservation and management of this highly exploited mollusc.
ISSN:1877-7252
1877-7260
DOI:10.1007/s12686-013-0085-4