Development of novel microsatellite markers for Cymodocea rotundata Ehrenberg (Cymodoceaceae), a pioneer seagrass species widely distributed in the Indo-Pacific

Genetic diversity and population genetic structure are key components of seagrass resilience and contribute to an understanding of its conservation and management. We isolated 29 polymorphic microsatellite (SSR) markers from a widely distributed pioneer seagrass, Cymodocea rotundata, by two methods;...

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Bibliographic Details
Published in:Conservation genetics resources 2014-03, Vol.6 (1), p.135-138
Main Authors: Arriesgado, Dan M, Nakajima, Yuichi, Matsuki, Yu, Lian, Chunlan, Nagai, Satoshi, Yasuike, Motoshige, Nakamura, Yoji, Fortes, Miguel D, Uy, Wilfredo H, Campos, Wilfredo L, Nakaoka, Masahiro, Nadaoka, Kazuo
Format: Article
Language:English
Subjects:
alleles
Animal Genetics and Genomics
Biodiversity
Biomedical and Life Sciences
Conservation Biology/Ecology
Cymodocea rotundata
Cymodoceaceae
Ecology
Evolutionary Biology
Genetic diversity
Genetic markers
Genetic structure
genetic variation
Heterozygosity
Life Sciences
loci
Microsatellite Letters
microsatellite repeats
Microsatellites
Next-generation sequencing
Plant Genetics and Genomics
Population genetics
seagrasses
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Summary:Genetic diversity and population genetic structure are key components of seagrass resilience and contribute to an understanding of its conservation and management. We isolated 29 polymorphic microsatellite (SSR) markers from a widely distributed pioneer seagrass, Cymodocea rotundata, by two methods; next generation sequencing and compound SSR marker isolation. Twenty-nine markers had 2–14 alleles per locus, and the observed and expected heterozygosity ranged from 0 to 0.688, and 0.113 to 0.770, respectively. These loci will facilitate investigation of the genetic diversity and population genetic connectivity and structure of C. rotundata.
ISSN:1877-7252
1877-7260
DOI:10.1007/s12686-013-0025-3