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Generic high-capacity protein capture and release by pH control
Techniques for immobilization and release of proteins are of general interest but challenging to develop. Here we show a new method for high-capacity (several μg cm −2 ) immobilization of proteins in polyelectrolyte brushes by multivalent hydrogen bonds. Upon increasing pH, the proteins are fully re...
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Published in: | Chemical communications (Cambridge, England) England), 2020-06, Vol.56 (44), p.5889-5892 |
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Main Authors: | , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | Techniques for immobilization and release of proteins are of general interest but challenging to develop. Here we show a new method for high-capacity (several μg cm
−2
) immobilization of proteins in polyelectrolyte brushes by multivalent hydrogen bonds. Upon increasing pH, the proteins are fully released with preserved structure and activity.
A new and generic method for protein capture-release by polyelectrolyte brushes and pH control. |
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ISSN: | 1359-7345 1364-548X 1364-548X |
DOI: | 10.1039/d0cc01250e |