Use of whole‐body cryosectioning and desorption electrospray ionization mass spectrometry imaging to visualize alkaloid distribution in poison frogs

Ambient mass spectrometry is useful for analyzing compounds that would be affected by other chemical procedures. Poison frogs are known to sequester alkaloids from their diet, but the sequestration pathway is unknown. Here, we describe methods for whole‐body cryosectioning of frogs and use desorptio...

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Bibliographic Details
Published in:Journal of mass spectrometry. 2020-06, Vol.55 (6), p.e4520-n/a
Main Authors: Jeckel, Adriana M., Matsumura, Kunihiro, Nishikawa, Keisuke, Morimoto, Yoshiki, Saporito, Ralph A., Grant, Taran, Ifa, Demian R.
Format: Article
Language:English
Subjects:
Alkaloids
Alkaloids - analysis
Ambient Analysis
Amphibia
Amphibian Venoms - analysis
Amphibians
Animals
Anura - physiology
chemical defense
Cryoultramicrotomy - methods
Dendrobatidae
Desorption
Distribution
Electrospraying
Frogs
histology
Imaging techniques
Ionization
Ions
Mass spectrometry
Mass spectroscopy
Oral administration
Scientific imaging
Spectrometry, Mass, Electrospray Ionization - methods
Spectroscopy
Tissue Distribution
Whole Body Imaging - methods
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Summary:Ambient mass spectrometry is useful for analyzing compounds that would be affected by other chemical procedures. Poison frogs are known to sequester alkaloids from their diet, but the sequestration pathway is unknown. Here, we describe methods for whole‐body cryosectioning of frogs and use desorption electrospray ionization mass spectrometry imaging (DESI‐MSI) to map the orally administered alkaloid histrionicotoxin 235A in a whole‐body section of the poison frog Dendrobates tinctorius. Our results show that whole‐body cryosectioning coupled with histochemical staining and DESI‐MSI is an effective technique to visualize alkaloid distribution and help elucidate the mechanisms involved in alkaloid sequestration in poison frogs.
ISSN:1076-5174
1096-9888
DOI:10.1002/jms.4520