Validation of non‐invasive transcutaneous measurement for glomerular filtration rate in lean and obese C57BL/6J mice

• Published in: Nephrology (Carlton, Vic.) Vic.), 2020-07, Vol.25 (7), p.575-581
• Main Authors: Mullins, Thomas P., Tan, Wei Sheng, Carter, David A., Gallo, Linda A.
• Format: Article
• Language: English
• Subjects: Animals; Body Weight - physiology; Diagnostic Techniques, Urological - instrumentation; Diet, High-Fat; Disease Progression; Fluoresceins - pharmacokinetics; Fluorescent Dyes - pharmacokinetics; Glomerular Filtration Rate; High fat diet; kidney; Kidney diseases; Kidney Function Tests - methods; Metabolic Clearance Rate; Mice; Mice, Inbred C57BL; Obesity; Oligosaccharides - pharmacokinetics; Plasma; Polycystic kidney; Polycystic Kidney Diseases - diagnosis; Polycystic Kidney Diseases - metabolism; Procedures and Techniques Utilization; Renal Elimination; renal function; Reproducibility of Results; rodents; Solitary Kidney - diagnosis; Solitary Kidney - metabolism; technique
• ISSN: 1320-5358; 1440-1797
• DOI: 10.1111/nep.13713

Aim The measurement of glomerular filtration rate (GFR) in experimental rodents is pivotal to understanding the progression of kidney disease and benefits of treatment strategies. A non‐invasive clearance device has been developed, which measures transcutaneous decay of injected FITC‐sinistrin in conscious rodents. The technique was validated against the well‐established plasma clearance method in the same mice, but on consecutive days, using only models of uninephrectomy and polycystic kidney disease. We aimed to validate this widely used technique in the same lean or obese mice, at the same time. Methods Five‐week‐old male C57BL/6J mice were randomised to a high fat diet (n = 12) or normal diet (n = 11) for 10 weeks. Transcutaneous and plasma clearance of FITC‐sinistrin were measured simultaneously in each mouse. Results In lean mice, there was a positive correlation between transcutaneous and plasma derived GFR (P