Gold nanoparticles enhance immune responses in mice against recombinant classical swine fever virus E2 protein

Objective To create a novel subunit vaccine that used AuNPs as carriers to enhance immune responses in mice against recombinant classical swine fever virus E2 protein (CSFV E2). Results Gold nanoparticles (AuNPs) were successfully coupled to the E2 protein and formed stable particle complexes called...

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Bibliographic Details
Published in:Biotechnology letters 2020-07, Vol.42 (7), p.1169-1180
Main Authors: Li, Yafei, Jin, Qianyue, Ding, Peiyang, Zhou, Wen, Chai, Yongxiao, Li, Xufeng, Wang, Yao, Zhang, Gaiping
Format: Article
Language:English
Subjects:
Animals
Antibodies
Antibodies, Viral - blood
Antibodies, Viral - immunology
Antigen-presenting cells
Antigens
Applied Microbiology
Biochemistry
Biomedical and Life Sciences
Biotechnology
Cytokines
Diarrhea
E2 protein
Female
Fever
Gold
Gold - chemistry
Hepatitis C
Hog cholera
Immune response
Immune system
Immunogenicity
Interleukin 10
Life Sciences
Lymphocytes
Metal Nanoparticles - chemistry
Mice
Mice, Inbred BALB C
Microbiology
Nanoparticles
Original Research Paper
Phagocytosis
Proteins
RAW 264.7 Cells
Swine
Vaccines
Viral Envelope Proteins - immunology
Viral Vaccines - chemistry
Viral Vaccines - immunology
Viruses
γ-Interferon
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Summary:Objective To create a novel subunit vaccine that used AuNPs as carriers to enhance immune responses in mice against recombinant classical swine fever virus E2 protein (CSFV E2). Results Gold nanoparticles (AuNPs) were successfully coupled to the E2 protein and formed stable particle complexes called E2 conjugated AuNPs (E2–AuNPs). In vitro studies have shown that the E2–AuNPs complex has the same immunogenicity as the E2 protein, and AuNPs can promote the phagocytosis of the E2 protein by antigen-presenting cells (APCs). In vivo results of BALB/c mice showed that the antibody levels, lymphocyte proliferation index, IFN-γ and IL-10 cytokines induced by E2–AuNPs were relatively higher than those of E2 or AuNPs group. Conclusions This finding demonstrated the potential of using AuNPs as a carrier to enhance the body's immune response for developing CSFV subunit vaccines. This model also contributes to the development of other flavivirus subunit vaccines, such as hepatitis C virus (HCV) and bovine viral diarrhea virus (BVDV).
ISSN:0141-5492
1573-6776
DOI:10.1007/s10529-020-02853-w